Comparison of radioimmunoassay and enzyme immunoassay kits for detection of Legionella pneumophila serogroup 1 antigen in both concentrated and nonconcentrated urine samples.

Journal of Clinical Microbiology
J DomínguezV Ausina

Abstract

We evaluated a commercial enzyme immunoassay (EIA) kit for detection of Legionella pneumophila serogroup 1 soluble antigen by comparing it to radioimmunoassay (RIA), using both concentrated and nonconcentrated urine samples. The sensitivity of EIA was 67.4% in nonconcentrated urine samples and 82.6% in concentrated urine samples. The sensitivity of RIA was 60.9% and 84.8% in nonconcentrated and concentrated urine samples, respectively. Our study indicates that the sensitivity and specificity of EIA are comparable to those of RIA, and that concentrating the antigen by selective ultrafiltration increases sensitivity for both EIA and RIA, with no significant decrease in specificity.

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Citations

Jul 5, 2002·Clinical Microbiology Reviews·Barry S FieldsRichard E Besser
Dec 22, 2006·Expert Review of Anti-infective Therapy·Carolina Garcia-Vidal, Jordi Carratalà
Oct 14, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J AlmedaV Ausina
Feb 15, 2002·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M SabriàC Rey-Joly
Jan 15, 2003·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N SopenaL Matas
Sep 12, 2018·Toxins·Camila Dias-LopesLiza Felicori
Aug 6, 2005·Journal of Clinical Microbiology·Almudena RojasJoaquín Ruiz

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