PMID: 8946567Nov 1, 1996Paper

Comparison of rat epididymal sperm counts by IVOS HTM-IDENT and hemacytometer

Reproductive Toxicology
L F StraderS D Perreault

Abstract

Epididymal sperm counts, a common measurement in male reproductive toxicity studies, are routinely determined using a hemacytometer. Recently, computer assisted methods for automated sperm counts have been developed. In the present study we evaluated an automated system, the TOX IVOS (Hamilton Thorne Research, Beverly, MA) HTM-IDENT option, that utilizes a DNA-specific stain and fluorescence illumination to identify sperm for enumeration. Cauda and caput epididymal sperm counts were determined in 48 adult male Sprague-Dawley rats, using both the hemacytometer and HTM-IDENT. The mean hemacytometer and HTM-IDENT counts (+/- SD) were 250 +/- 43 and 254 +/- 52 million, respectively, for cauda sperm, and 123 +/- 13 and 127 +/- 18 million, respectively, for caput sperm. The average coefficient of variation using the hemacytometer was 13.8% as compared to 17.3% for the HTM-IDENT. Comparison of the machine count and a visual count from the Display Statics screen of the HTM-IDENT indicated that when two or more sperm heads touched or overlapped, the machine counted them as one. Manual (visual) and machine counts when compared over a range of nine concentrations from 3.7 to 47.8 million/mL differed by 4 to 12% at the lowest to highest co...Continue Reading

References

Sep 1, 1978·Journal of Reproduction and Fertility·G W RobbG J Killian
Aug 1, 1986·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R P Amann, W E Berndtson
Dec 1, 1985·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·W F BlazakB E Stewart

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Citations

Oct 6, 1997·Reproductive Toxicology·R E LinderN L Roberts
Feb 10, 2009·Journal of Toxicology and Environmental Health. Part a·D P ArfstenM Y-V Bekkedal
Nov 21, 2007·The Journal of Reproduction and Development·Ehn-Kyoung ChoiMasamichi Kurohmaru
Apr 16, 2017·Reproductive Toxicology·Peter MorfeldMichela Mastrantonio
Apr 24, 2010·Human & Experimental Toxicology·Hassan MalekinejadFereshteh Dardmeh

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