Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples.

World Journal of Gastroenterology : WJG
Ming ShiWei-Jia Xu

Abstract

To compare the clinical performance of a real-time PCR assay with the COBAS Amplicor Hepatitis B Virus (HBV) Monitor test for quantitation of HBV DNA in serum samples. The reference sera of the Chinese National Institute for the Control of Pharmaceutical and Biological Products and the National Center for Clinical Laboratories of China, and 158 clinical serum samples were used in this study. The linearity, accuracy, reproducibility, assay time, and costs of the real-time PCR were evaluated and compared with those of the Cobas Amplicor test. The intra-assay and inter-assay variations of the real-time PCR ranged from 0.3% to 3.8% and 1.4% to 8.1%, respectively. The HBV DNA levels measured by the real-time PCR correlated very well with those obtained with the COBAS Amplicor test (r = 0.948). The real-time PCR HBV DNA kit was much cheaper and had a wider dynamic range. The real-time PCR assay is an excellent tool for monitoring of HBV DNA levels in patients with chronic hepatitis B.

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Citations

Jul 29, 2015·Journal of Virological Methods·Moyra Machado PortilhoLivia Melo Villar
Jun 15, 2011·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Hai-Yang XieShu-Sen Zheng
Aug 5, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yong-Zhong WangGuo-Xiang Wu
Jan 6, 2012·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Yong-Zhong WangKe-Zhuan Zhu
Feb 24, 2012·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Ming ShiLu Xing

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