Comparison of real-time polymerase chain reaction and conventional biochemical methods for identification of Mycobacterium chelonae-Mycobacterium abscessus group to the species level

Diagnostic Microbiology and Infectious Disease
Nora GuarinNiaz Banaei

Abstract

The Mycobacterium chelonae-Mycobacterium abscessus group (MCAG) is the most common cause of infections because of rapidly growing mycobacteria. Rapid identification of MCAG to the species level is essential for choosing empiric antibiotic treatment and for public health measures. In this study, we compared the performance of a single-tube multiplex, real-time polymerase chain reaction (PCR) assay to 3 biochemical tests for species-level identification of 46 MCAG isolates. We show that real-time PCR provides the most accurate results for rapid species-level identification of MCAG.

References

Jan 19, 1999·Annual Review of Microbiology·R J WallaceD E Griffith
Oct 30, 2001·Journal of Clinical Microbiology·M A YakrusB Metchock
Jul 10, 2004·Journal of Clinical Microbiology·L SedlacekF C Bange
Feb 6, 2007·American Journal of Respiratory and Critical Care Medicine·David E GriffithUNKNOWN Infectious Disease Society of America
Apr 18, 2008·Journal of Clinical Microbiology·Loree C HellerRay H Widen

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Citations

Apr 13, 2013·Journal of Medical Microbiology·Nancy E RolfeStephanie P Taylor
Mar 21, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C ArnoldJ G Magee
Feb 13, 2015·Anais Brasileiros De Dermatologia·Renan LageAdilson da Costa

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