Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell-free body cavity effusion for the diagnosis of feline infectious peritonitis

Journal of Feline Medicine and Surgery
Stephanie J DoengesKatrin Hartmann

Abstract

Objectives Diagnosis of feline infectious peritonitis (FIP) remains challenging, especially in cats without effusions. The objective of this study was to evaluate the sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction (RT-PCR) detecting feline coronavirus (FCoV) RNA in peripheral blood mononuclear cells (PBMCs) and serum in comparison with the same real-time RT-PCR in cell-free body cavity effusion. Methods This prospective case-control study included 92 cats. Forty-three cats had a definitive diagnosis of FIP, established either by histopathological examination (n = 28) or by positive immunofluorescence staining of FCoV antigen in macrophages of effusions (n = 11), or by both methods (n = 4). Forty-nine control cats had other diseases but similar clinical signs. Real-time RT-PCR was performed on PBMCs of 37 cats (21 cats with FIP, 16 controls), on serum of 51 cats (26 cats with FIP, 25 controls) and on cell-free body cavity effusion of 69 cats (36 cats with FIP, 33 controls). Sensitivity, specificity, positive and negative predictive value, including 95% confidence intervals (CI), were calculated. Results Real-time RT-PCR of PBMCs, serum and cell-free body cavity effusion showed a specif...Continue Reading

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Citations

Jan 23, 2017·Journal of Virological Methods·Angelica StranieriSaverio Paltrinieri
Feb 27, 2018·Journal of Feline Medicine and Surgery·Séverine Tasker
Jan 30, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sandra FeltenKaspar Matiasek
Jun 11, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Laura SanglKatrin Hartmann
Nov 17, 2019·Viruses·Sandra Felten, Katrin Hartmann
May 10, 2017·The Journal of Veterinary Medical Science·Michihito TagawaKazuro Miyahara

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Methods Mentioned

BETA
biopsy
density gradient centrifugation
PCR

Software Mentioned

Graph Pad Prism

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