Comparison of red-shifted firefly luciferase Ppy RE9 and conventional Luc2 as bioluminescence imaging reporter genes for in vivo imaging of stem cells.

Journal of Biomedical Optics
Yajie LiangJeff W M Bulte

Abstract

One critical issue for noninvasive imaging of transplanted bioluminescent cells is the large amount of light absorption in tissue when emission wavelengths below 600 nm are used. Luciferase with a red-shifted spectrum can potentially bypass this limitation. We assessed and compared a mutant of firefly luciferase (Ppy RE9, PRE9) against the yellow luciferase luc2 gene for use in cell transplantation studies. C17.2 neural stem cells expressing PRE9-Venus and luc2-Venus were sorted by flow cytometry and assessed for bioluminescence in vitro in culture and in vivo after transplantation into the brain of immunodeficient Rag2-/- mice. We found that the luminescence from PRE9 was stable, with a peak emission at 620 nm, shifted to the red compared to that of luc2. The emission peak for PRE9 was pH-independent, in contrast to luc2, and much less affected by tissue absorbance compared to that of luc2. However, the total emitted light radiance from PRE9 was substantially lower than that of luc2, both in vitro and in vivo. We conclude that PRE9 has favorable properties as compared to luc2 in terms of pH independence, red-shifted spectrum, tissue light penetration, and signal quantification, justifying further optimization of protein expres...Continue Reading

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J R de WetM DeLuca
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D YandavaE Y Snyder
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·H MizuguchiT Hayakawa
Dec 1, 2001·Journal of Biomedical Optics·B W RiceM B Nelson
Feb 28, 2004·Stroke; a Journal of Cerebral Circulation·Dong-Eog KimRalph Weissleder
May 1, 2004·Bioscience, Biotechnology, and Biochemistry·Yoshihiro NakajimaYoshihiro Ohmiya
Aug 30, 2005·Analytical Biochemistry·Bruce R BranchiniAldo Roda
Sep 22, 2005·Nature Reviews. Cancer·Rosa A CardoneStephan J Reshkin
Jan 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hirotaka MasudaYasunori Yoshimura
Jul 27, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P WalczakJ W M Bulte
Oct 30, 2007·BMC Biotechnology·Camille Allera-MoreauAnne-Catherine Prats
Nov 13, 2007·Journal of Biomedical Optics·Tewfik MiloudGünter J Hämmerling
Nov 17, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Zachary LoveZhenghong Lee
Mar 29, 2008·Methods in Molecular Biology·Yuan LinStanton L Gerson
Nov 7, 2008·Methods in Molecular Biology·Ren-Jang Lin
Aug 18, 2009·Methods in Molecular Biology·Kitchener D WilsonJoseph C Wu
Sep 15, 2009·Analytical Biochemistry·Bruce R BranchiniMartin A Pule
Nov 26, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Laura MezzanotteAldo Roda
Sep 14, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·K A ChuangY C Kuo
Oct 12, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Stacey Cromer BermanPiotr Walczak

❮ Previous
Next ❯

Citations

Apr 30, 2013·Biomaterials·Yajie LiangJeff W M Bulte
May 7, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·P Stephen PatrickKevin M Brindle
Sep 17, 2015·Applied Microbiology and Biotechnology·K von der HaarC Blume
Sep 2, 2014·Frontiers in Cellular Neuroscience·Markus AswendtAnnette Tennstaedt
Jul 23, 2016·Frontiers in Oncology·Tingting XuSteven Ripp
Mar 17, 2015·Nuclear Medicine and Molecular Imaging·Jung Eun KimByeong-Cheol Ahn
May 17, 2019·Neurophotonics·Markus AswendtMathias Hoehn
Feb 6, 2020·The Journal of Antimicrobial Chemotherapy·Samantha A MichaelsKayode K Ojo
Dec 15, 2019·Nature Communications·Alejandro Sarrion-PerdigonesKoen J T Venken
Jul 5, 2017·Cellular and Molecular Life Sciences : CMLS·Piotr JurgielewiczOmer Aras
Nov 8, 2020·The Journal of Biological Chemistry·Giulia BandiniJohn Samuelson
Jul 9, 2017·Analytical Biochemistry·Bruce R BranchiniMartha J Grossel
Jan 8, 2021·The Journal of Biological Chemistry·Giulia BandiniJohn Samuelson
May 10, 2017·Current Opinion in Biotechnology·Kazushi Suzuki, Takeharu Nagai
Jul 28, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Francesco AmadeoArthur Taylor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.