Comparison of refolding activities between nanogel artificial chaperone and GroEL systems

International Journal of Biological Macromolecules
Wakiko AsayamaK Akiyoshi

Abstract

The chaperone-like activity of a nanogel of cholesteryl group-bearing pullulan (CHP) was compared with that of GroEL for refolding acid-denatured green fluorescent protein (GFP). The refolding of denatured GFP was carried out by dilution of acid-denatured GFP in the presence of the nanogel or GroEL. GFP fluorescence was increasingly repressed with increases in the concentration of the CHP nanogel or GroEL added to the dilution buffer. The concentrations of 50% inhibition of recovery of GFP fluorescence were 0.03 microM (GroEL) and 0.08 microM (CHP nanogel), respectively. The refolding was resumed by the addition of ATP into the GroEL (0.20 microM) system or by the addition of methyl-beta-cyclodextrin into the nanogel (0.20 microM) system. In the nanogel-GFP system, about 90% of the intensity was recovered within 10 min. The half time (t(1/2)) for refolding in the CHP nanogel system (36 s) is almost equal to that of the natural chaperone GroEL-GroES system.

References

Aug 31, 1981·Biochemical and Biophysical Research Communications·S H Bokman, W W Ward
Apr 1, 1997·Protein Science : a Publication of the Protein Society·W A Fenton, A L Horwich
May 9, 1997·The Journal of Biological Chemistry·Y MakinoM Yoshida
Feb 26, 1998·Cell·B Bukau, A L Horwich
Oct 1, 1996·Nature Biotechnology·F YangG N Phillips
Jul 17, 1998·Current Opinion in Biotechnology·E D B Clark
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
Oct 6, 1998·Annual Review of Biochemistry·P B SiglerA L Horwich
Jul 20, 1999·The Journal of Biological Chemistry·C SakikawaM Yoshida
Oct 6, 1999·Current Opinion in Biotechnology·F Baneyx
Feb 16, 2000·Biochemical and Biophysical Research Communications·F MotojimaM Yoshida
Dec 19, 2001·Current Biology : CB·R J Ellis
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
May 20, 2004·Molecular Cell·Taro UenoTakashi Funatsu
Dec 4, 2004·Biotechnology Progress·Heikki Lanckriet, Anton P J Middelberg
Oct 10, 2006·International Journal of Biological Macromolecules·Razieh Yazdanparast, Reza Khodarahmi

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Citations

Apr 7, 2011·Social Cognitive and Affective Neuroscience·James K RillingTimothy E Behrens
Jul 4, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Urara HasegawaKazunari Akiyoshi
Aug 12, 2015·Chemical Reviews·Yulin LiHelena Tomás
Mar 6, 2012·International Journal of Pharmaceutics·Maria D Moya-OrtegaThorsteinn Loftsson
Dec 2, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Gabriel S LongoIgal Szleifer
Sep 18, 2018·Advanced Healthcare Materials·Yoshihide HashimotoKazunari Akiyoshi
Sep 14, 2010·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Yoshihiro Sasaki, Kazunari Akiyoshi
Oct 10, 2013·The Journal of Dairy Research·Milagro Borràs LlopisMaria Pilar Molina Pons
Mar 9, 2011·Macromolecular Bioscience·Yoshihiro SasakiKazunari Akiyoshi
Mar 18, 2009·Macromolecular Bioscience·Yayoi OzawaKazunari Akiyoshi
Jun 6, 2020·Biomaterials·Orakan Hanpanich, Atsushi Maruyama
Apr 28, 2020·Bioconjugate Chemistry·Tomoki Nishimura, Kazunari Akiyoshi

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