Comparison of responses to electrical stimulation and whisker deflection using two different voltage-sensitive dyes in mouse barrel cortex in vivo.

The Journal of Membrane Biology
Eugene F Civillico, Diego Contreras

Abstract

We examined the spatial structure of noise in optical recordings made with two commonly used voltage-sensitive dyes (RH795 and RH1691) in mouse barrel cortex in vivo, and determined that the signal-to-noise ratio of the two dyes was comparable when averaging over barrel-sized areas, or at single pixels distant from large blood vessels. We examined the spatiotemporal development of whisker- and electrically-evoked optical responses by quantifying the area of activated cortical surface as a function of time. Whisker and electrical stimuli activated cortical areas between 0.2-2.0 mm(2) depending on intensity. More importantly, both types of activation recruited cortical area at similar rates and showed a linear relationship between the maximal activated area and the peak rate of increase of the activated area. We propose a general rule of supragranular cortical activation in which the initial spreading speed of the response determines the total activated area, independent of the type of activation. Finally, despite comparable single-response kinetics, we observed greater paired-pulse depression of whisker-evoked responses relative to electrically-evoked responses.

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Citations

Apr 1, 2008·Cerebral Cortex·Bashir AhmedPer E Roland
Nov 11, 2008·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Jian-Young Wu Chuan Zhang
Feb 14, 2018·Journal of Neural Engineering·Jonathan A N Fisher, Iryna Gumenchuk
Oct 21, 2016·Journal of Neurophysiology·Clarissa J WhitmireGarrett B Stanley
Feb 14, 2008·Nature Reviews. Neuroscience·Jason N D Kerr, Winfried Denk
Mar 31, 2006·Journal of Neurophysiology·Eugene F Civillico, Diego Contreras
Jun 23, 2006·Journal of Neurophysiology·Krista M RodgersDaniel S Barth
Feb 26, 2016·Journal of Neurophysiology·Sandrine Chemla, Frédéric Chavane
Sep 19, 2018·Scientific Reports·Shany Nivinsky Margalit, Hamutal Slovin
Jun 17, 2021·Nature Communications·Taylor H NewtonHenry Markram

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Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.