Comparison of results obtained with Amplicor HIV-1 DNA PCR test version 1.5 using 100 versus 500 microliters of whole blood

Journal of Clinical Microbiology
Estelle Piwowar-ManningJ Brooks Jackson

Abstract

The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 microl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 microl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.

References

Sep 16, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jeffrey J GermerJoseph D C Yao
Sep 14, 2007·American Journal of Obstetrics and Gynecology·Tracy L CreekNathan Shaffer

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Citations

Jul 16, 2009·Journal of Tropical Pediatrics·A L SlogroveM M Esser
Mar 21, 2012·BMC Research Notes·Steven BaveewoRhoda K Wanyenze
Dec 27, 2015·Journal of Virological Methods·Sokhna Bousso GueyeCoumba Touré-Kane

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