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Comparison of Ribosomal RNA Removal Methods for Transcriptome Sequencing Workflows in Teleost Fish

Animal Biotechnology

Jan 7, 2016

Jason Abernathy, Ken Overturf

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Abstract

RNA sequencing (RNA-Seq) is becoming the standard for transcriptome analysis. Removal of contaminating ribosomal RNA (rRNA) is a priority in the preparation of libraries suitable for sequencing. These methods have been well documented in mammals but typically require some optimization f...read more

Mentioned in this Paper

Vertebrates
Oncorhynchus mykiss antigen
Teleost fish
Sequence Determinations, RNA
Nucleic Acid Hybridization Procedure
Nucleic Acid Sequencing
Ribosomal RNA
Gene Expression
Evaluation
Sequencing
4
25
Paper Details
References
  • References11
  • Citations2
12
  • References11
  • Citations2

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Comparison of Ribosomal RNA Removal Methods for Transcriptome Sequencing Workflows in Teleost Fish

Animal Biotechnology

Jan 7, 2016

Jason Abernathy, Ken Overturf

PMID: 26732342

DOI: 10.1080/10495398.2015.1086365

Abstract

RNA sequencing (RNA-Seq) is becoming the standard for transcriptome analysis. Removal of contaminating ribosomal RNA (rRNA) is a priority in the preparation of libraries suitable for sequencing. These methods have been well documented in mammals but typically require some optimization f...read more

Mentioned in this Paper

Vertebrates
Oncorhynchus mykiss antigen
Teleost fish
Sequence Determinations, RNA
Nucleic Acid Hybridization Procedure
Nucleic Acid Sequencing
Ribosomal RNA
Gene Expression
Evaluation
Sequencing
4
25

Feeds With Similar Papers

Human Liver Analysis

Mapping the human liver by combining transcriptomics, spatial organization, and cell-cell interactions will facilitate the study of liver disease and the development of novel therapeutics. This feed includes new research on analysis of the human liver.

Human Liver Diversity Over a Lifespan

Mapping the human liver by combining transcriptomics, spatial organization, and cell-cell interactions will facilitate the study of liver disease and the development of novel therapeutics. This feed includes new research on analysis of the human liver.

Related Papers

Biochemical Society Transactions

Electrophoretic analysis of rainbow trout (Oncorhynchus mykiss) mucus

Biochemical Society TransactionsFebruary 1, 1996
A J HutchinsonU MacEvilly
Paper Details
References
  • References11
  • Citations2
12
  • References11
  • Citations2

Download from

Publisher
PubMed
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