Comparison of RNA- and DNA-based bacterial communities in a lab-scale methane-degrading biocover

Applied Microbiology and Biotechnology
Tae Gwan KimKyung-Suk Cho

Abstract

Methanotrophs must become established and active in a landfill biocover for successful methane oxidation. A lab-scale biocover with a soil mixture was operated for removal of methane and nonmethane volatile organic compounds, such as dimethyl sulfide (DMS), benzene (B), and toluene (T). The methane elimination capacity was 211±40 g m(-2) d(-1) at inlet loads of 330-516 g m(-2) d(-1). DMS, B, and T were completely removed at the bottom layer (40-50 cm) with inlet loads of 221.6±92.2, 99.6±19.5, and 23.4±4.9 mg m(-2) d(-1), respectively. The bacterial community was examined based on DNA and RNA using ribosomal tag pyrosequencing. Interestingly, methanotrophs comprised 80% of the active community (RNA) while 29% of the counterpart (DNA). Types I and II methanotrophs equally contributed to methane oxidation, and Methylobacter, Methylocaldum, and Methylocystis were dominant in both communities. The DNA vs. RNA comparison suggests that DNA-based analysis alone can lead to a significant underestimation of active members.

References

Jan 1, 1994·Archives of Microbiology·R Wagner
Mar 21, 1998·Applied and Environmental Microbiology·A FelskeA D Akkermans
Jun 11, 1998·Microbial Ecology·A Felske, A D L Akkermans
Apr 1, 2000·Applied and Environmental Microbiology·J A KlappenbachT M Schmidt
Mar 2, 2002·Applied and Environmental Microbiology·Markus G WeinbauerManfred G Höfle
Nov 27, 2002·Applied and Environmental Microbiology·Robert A KanalyKazuya Watanabe
Dec 6, 2003·Environmental Science & Technology·Charlotte SchuetzPeter Kjeldsen
Aug 23, 2005·Journal of the Air & Waste Management Association·Charlotte Scheutz, Peter Kjeldsen
Jan 27, 2007·Genome Research·Daniel H HusonStephan C Schuster
Oct 4, 2007·International Journal of Systematic and Evolutionary Microbiology·Jongsik ChunYoung-Woon Lim
Mar 4, 2008·Environmental Microbiology·Julia GebertLevente Bodrossy
Nov 14, 2008·Nucleic Acids Research·J R ColeJ M Tiedje
May 26, 2009·Journal of Environmental Quality·Charlotte ScheutzPeter Kjeldsen
Jul 9, 2009·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Charlotte ScheutzKurt Spokas
Dec 5, 2009·Journal of Hazardous Materials·Kyung-Eun MoonKyung-Suk Cho
Dec 17, 2009·FEMS Microbiology Reviews·Dieter Haas
Mar 20, 2010·FEMS Microbiology Reviews·Jeremy D SemrauSukhwan Yoon
May 4, 2010·International Journal of Systematic and Evolutionary Microbiology·Hiroyuki IguchiYasuyoshi Sakai
Nov 16, 2010·Waste Management·Julia GebertAlexandre R Cabral
Mar 28, 2012·Journal of Microbiology and Biotechnology·Tae Gwan KimKyung-Suk Cho
Oct 1, 2009·Environmental Microbiology Reports·Qiongfen QiuYahai Lu

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Citations

Oct 12, 2012·Applied Microbiology and Biotechnology·Tae Gwan KimKyung-Suk Cho
Nov 30, 2013·Applied Microbiology and Biotechnology·Tae Gwan KimKyung-Suk Cho
May 16, 2014·Journal of Industrial Microbiology & Biotechnology·Kyung-Eun MoonKyung-Suk Cho
May 30, 2014·Bioprocess and Biosystems Engineering·Jung-Yeol LeeKyung-Suk Cho
Jan 13, 2016·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Yun-Yeong LeeKyung-Suk Cho
Mar 10, 2016·Bioprocess and Biosystems Engineering·Yun-Yeong LeeKyung-Suk Cho
Jul 6, 2015·Journal of Bioscience and Bioengineering·Eun-Hee LeeKyung-Suk Cho
Sep 2, 2015·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Jeonghee YunKyung-Suk Cho
Jun 21, 2015·Bioresource Technology·Yun-Yeong LeeKyung-Suk Cho
Jun 22, 2014·Applied Microbiology and Biotechnology·Kevin J PortuneCarmen Gabaldón
Jan 15, 2014·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Tae Gwan KimKyung-Suk Cho
Jul 19, 2016·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Yun-Yeong LeeKyung-Suk Cho
Jan 6, 2018·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Bo-Min KiKyung-Suk Cho
Feb 23, 2018·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Bo-Min KiKyung-Suk Cho
Mar 5, 2020·World Journal of Microbiology & Biotechnology·Richa SharmaShilpi Sharma
Dec 18, 2020·Journal of Applied Microbiology·S-Y Jeong, T G Kim
Sep 4, 2018·The Science of the Total Environment·Hu LiYong-Guan Zhu
Nov 2, 2021·Frontiers in Microbiology·Sarah I LeichtyKirsten S Hofmockel

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