PMID: 590277Nov 4, 1977

Comparison of RNA and DNA synthesis, spontaneous and PHa induced, between blood lymphoid cells of newborn infants, older infants, and adults. A study of scintillation counting and by autoradiography

European Journal of Pediatrics
Gregor PrindullJ M Yoffey

Abstract

Spontaneous and PHA induced RNA and DNA synthesis was measured qualitatively by autoradiography and quantitatively by scintillation counting in blood lymphoic cells of newborn infants, older infants, and adults. Spontaneous RNA synthesis was found in transitional cells, large phagocytic lymphoid cells and lymphocytes. Transitional cells and phagocytic lymphoid cells also synthesized DNA spontaneously. Quantitatively, spontaneous RNA synthesis was active in both newborn infants and in adults, but significantly less so in older infants. PHA stimulation for 18 h increased RNA synthesis significantly in blood lymphoid cells of newborn infants and adults, and to a much lesser but still significant degree in older infants. Spontaneous DNA synthesis was significantly greater in newborn infants than in older infants and adults. PHA stimulation for 18 h had no effect on thymidine incorporation in any of the groups studied.

References

Mar 22, 1975·Lancet·E Diaz-JouanenR G Strickland
May 1, 1975·The Journal of Pediatrics·M Rola-PlezczynskiJ A Bellanti
May 1, 1975·The American Journal of Medicine·E Diaz-JauanenR C Williams
Feb 22, 1975·British Medical Journal·V Y YuJ D Baum
Jun 1, 1975·Developmental Biology·R B MorrisD F Bainton
Jan 1, 1975·Biology of the Neonate·Gregor PrindullJ M Yoffey
Mar 1, 1974·Acta paediatrica Scandinavica·A G UgazioG R Burgio
Nov 6, 1965·Lancet·G C WinterJ M Yoffey
Jul 1, 1973·Cellular Immunology·W P FaulkJ M Yoffey
Apr 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B G PogoA E Mirsky
Aug 1, 1970·Blut·F Otto, D O Schmid
Jun 15, 1969·American Journal of Obstetrics and Gynecology·W R Jones
Oct 23, 1965·Nature·G J Johnson, P S Russell
May 1, 1965·Experimental Cell Research·R I WALKER, I FOWLER

Citations

Oct 12, 1978·European Journal of Pediatrics·Gregor Prindull, A Hespe
Jul 1, 1978·Acta paediatrica Scandinavica·Gregor PrindullN Meulen
Jun 20, 2007·Experimental Hematology·Gregor A Prindull, Eitan Fibach

Related Concepts

Autoradiography
DNA, Double-Stranded
Lymphoid Cells
Phagocytes
RNA
Scintillation Counting
Isolectins

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