PMID: 9650955Jul 3, 1998Paper

Comparison of Roche Cobas Amplicor Mycobacterium tuberculosis assay with in-house PCR and culture for detection of M. tuberculosis

Journal of Clinical Microbiology
B R EingR Ringelmann

Abstract

The new Roche Cobas Amplicor Mycobacterium tuberculosis assay, which is a semiautomated version of the manually performed Roche Amplicor M. tuberculosis test, was compared to culture and an IS6110-based in-house PCR protocol. A total of 1,681 specimens from 833 patients, including specimen types other than sputum, were tested in parallel by both the in-house PCR and the Cobas Amplicor M. tuberculosis assay. After we resolved discrepant PCR results, the sensitivity, specificity, and positive and negative predictive values for the Cobas Amplicor M. tuberculosis assay were 66.33, 99.71, 94.36, and 97.66%, respectively. The corresponding values for the in-house PCR were 91.08, 99.85, 97.87, and 99.37%, respectively. For culture- and smear-positive specimens, the sensitivity of the Cobas Amplicor M. tuberculosis test was 96.42% (in-house PCR, 100%). If only smear-negative sputum specimens were considered, the Cobas Amplicor M. tuberculosis assay exhibited a sensitivity of 45.45% (in-house PCR, 63.63%) relative to that of culture. With a modified protocol for DNA extraction (washing of samples plus ultrasonication), both PCR methods performed better with gastric aspirates than with sputum samples (sensitivity of the Cobas Amplicor M....Continue Reading

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Citations

Feb 27, 1999·The Pediatric Infectious Disease Journal·N NeuP Della-Latta
Dec 12, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Gomez-PastranaJ Navarro
May 6, 1999·Current Opinion in Pulmonary Medicine·S Gillespie
Nov 7, 2000·Journal of Clinical Pathology·S A Watterson, F A Drobniewski
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Jan 20, 2006·FEMS Immunology and Medical Microbiology·Aysen BayramIclal Balci
Nov 6, 2007·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Hermides PintoDiógenes Santiago Santos
Aug 31, 2012·Journal of Clinical Microbiology·Yukio HidaMasayuki Iwano
Jul 25, 2014·Journal of Clinical Microbiology·Vinod B PatelKeertan Dheda
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I C ShamputaF Portaels
Aug 31, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fernando OsoresCiro Maguiña
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Dec 10, 2003·Journal of Clinical Microbiology·Claudio Piersimoni, Claudio Scarparo
Feb 13, 1999·Journal of Clinical Microbiology·B A HannaA M Vannier

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