PMID: 11341928May 9, 2001Paper

Comparison of salt effects on the reactions of acetylcholinesterase with cationic and anionic inhibitors

Biochimica Et Biophysica Acta
V Tõugu, T Kesvatera

Abstract

The influence of inorganic salts on the inhibition of acetylcholinesterase by charged organophosphorous inhibitors has been studied. It has been shown that the salt effect on the reaction of acetylcholinesterase with anionic bis(p-nitrophenyl) phosphate is determined by the influence of added salts on the activity coefficient of the inhibitor. In contrast to the salt effects on the reaction of acetylcholinesterase with cationic compounds, it does not include contribution from the enzyme charges. The smaller salt effect in the case of anionic inhibitor can be explained assuming that the anionic inhibitor does not form a non-covalent complex with the enzyme before the phosphorylation step of the reaction. Comparison of salt effects on the substrate turnover showed that in the case of cholinesterases from natural sources they are larger than in the case of enzymes expressed in recombinant cell clones. The enhanced salt effects may result from post-translational modification of the enzyme.

References

May 2, 1979·European Journal of Biochemistry·R RabaJ Siigur
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
Nov 27, 1990·Biochemistry·H A Berman, K Leonard
Apr 4, 1995·Biochemistry·J AntosiewiczM K Gilson
Jun 1, 1994·European Journal of Biochemistry·V TõuguA Aaviksaar
Jun 11, 1993·Biochimica Et Biophysica Acta·V TõuguA Aaviksaa
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D R RipollJ L Sussman
Jan 15, 1996·European Journal of Biochemistry·P MassonO Lockridge
Nov 14, 1996·Biochimica Et Biophysica Acta·V Tõugu, T Kesvatera

❮ Previous
Next ❯

Citations

Sep 16, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Bożena BukowskaMaria Koter-Michalak
Mar 12, 2013·International Journal of Biological Macromolecules·Bożena SosnowskaBożena Bukowska
Sep 15, 2012·Biological & Pharmaceutical Bulletin·Anna JarominArkadiusz Kozubek
Sep 25, 2008·Toxicon : Official Journal of the International Society on Toxinology·L L AllumE A H Hall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.