Comparison of Sample Preparation and Determination of 60 Veterinary Drug Residues in Flatfish Using Liquid Chromatography-Tandem Mass Spectrometry

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Joohye KimJae-Ho Oh

Abstract

This study was performed to optimize the analytical method for multi-residues of 60 compounds in flatfish samples. Three sample preparation methods were tested to identify the optimal recovery conditions for target analytes. As a result, 10 mL of water/acetonitrile (1:4, v/v) was used to extract analytes from fish samples. For purification, C18 and 10 mL of acetonitrile saturated hexane were used to treat the samples. After evaporation and reconstitution, the fish samples were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. The proposed method was validated according to the CODEX guidelines (CAC/GL-71). Our results showed the recoveries of 73.2%-115% and coefficients of variation of 1.6%-22.1%. The limit of quantification was 0.0005-0.005 mg/kg in the fishery products. In analysis of real samples, no samples exceeded the limit of quantification. This analytical method can be used for multi-residue screening and confirmation of the residues of veterinary drugs in fishery products.

References

Aug 30, 2008·Journal of Chromatography. a·M M Aguilera-LuizAntonia Garrido Frenich
Sep 24, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ole E HeuerFrederick J Angulo
Oct 20, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xi XiaJianzhong Shen
Nov 9, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Omnia A IsmaielH Thomas Karnes
Aug 8, 2012·Journal of Chromatography. a·Xiaoqin WuJay Gan
Jan 25, 2013·Journal of Agricultural and Food Chemistry·Jaime Nácher-MestreFélix Hernández
Apr 9, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Scarlett BiselliLutz Hartig
Apr 12, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M GbylikJ Zmudzki
Apr 30, 2013·Journal of Chromatography. a·Kaklamanos GeorgeChristoph von Holst
Feb 6, 2014·Journal of Agricultural and Food Chemistry·Richard J FussellMatthew Sharman
Aug 16, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Leslie C Dickson
Jan 12, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Junghyun KimSang Beom Han
Sep 3, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Estelle DubreilDominique Pessel
Nov 30, 2016·The Science of the Total Environment·Hee-Young KimJeong-Eun Oh
Nov 25, 2017·Bioanalysis·Wanlong ZhouPerry G Wang
Jul 4, 2018·Frontiers in Microbiology·Claudio D MirandaMatthew R Lee

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