PMID: 9650980Jul 3, 1998Paper

Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR

Journal of Clinical Microbiology
B JaulhacJerome Etienne

Abstract

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.

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Citations

Nov 29, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S ParkerA Gajadhar
Apr 29, 2005·Journal of Periodontology·Guglielmo CampusGiancarlo Tonolo
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Feb 17, 2021·PloS One·Fernando Lázaro-PeronaUNKNOWN SARS-CoV-2 Working Group
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Sep 15, 2009·Journal of Microbiological Methods·Monika KernHans Helmut Niller
Aug 20, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K LeviK J Towner

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