Abstract
Exclusion of broiler batches, highly colonized with Campylobacter (>7.5 log10 colony-forming units/g), from the fresh poultry meat market might decrease the risk of human campylobacteriosis. The objective of this study was to compare different sample types (both at the farm and the slaughterhouse) and methods (direct culture, quantitative real-time polymerase chain reaction [qPCR], propidium monoazide [PMA]-qPCR) applied for the quantification of the Campylobacter colonization level. In addition, the applicability of the lateral flow-based immunoassay, Singlepath(®) Direct Campy Poultry test (Singlepath(®) test), was evaluated as a rapid method for the qualitative detection of Campylobacter in highly colonized broiler batches. Campylobacter counts differed significantly between sample types collected at farm level (cecal droppings, feces, boot swabs) and at slaughterhouse level (cecal content, fecal material from crates). Furthermore, comparison of Campylobacter counts obtained by different methods (direct culture, qPCR, PMA-qPCR) in cecal droppings revealed significant differences, although this was not observed for cecal-content samples. Evaluation of the Singlepath(®) test on cecal droppings and cecal-content samples reveale...Continue Reading
References
Nov 1, 1996·Research in Microbiology·D LintonJ Stanley
Oct 23, 1997·International Journal of Systematic Bacteriology·P VandammeS L On
Mar 5, 2003·Epidemiology and Infection·N J SternM T Musgrove
Feb 10, 2004·Applied and Environmental Microbiology·Knut RudiKjetill S Jakobsen
Feb 13, 2004·Epidemiology and Infection·L HermanL De Zutter
Feb 16, 2006·International Journal of Food Microbiology·Hanne RosenquistBjarke B Christensen
Apr 28, 2007·Veterinary Microbiology·G RasschaertL De Zutter
Sep 29, 2007·Journal of Food Protection·J A CasonN A Cox
Dec 3, 2008·Applied and Environmental Microbiology·Twan van GerweDon Klinkenberg
Jan 31, 2009·International Journal of Food Microbiology·J D Greig, A Ravel
Aug 14, 2009·International Journal of Food Microbiology·M J NautaN M Bolder
Jun 22, 2010·Applied and Environmental Microbiology·M H JosefsenJ Hoorfar
Jul 1, 2010·Foodborne Pathogens and Disease·Sara Monteiro PiresTine Hald
Sep 8, 2010·Journal of Food Protection·Imca SampersMieke Uyttendaele
May 9, 2012·Veterinary Microbiology·J D RodgersF A Clifton-Hadley
Aug 16, 2012·Epidemiology and Infection·E V TaylorR V Tauxe
Aug 31, 2012·Zoonoses and Public Health·A B VidalF Clifton-Hadley
Sep 20, 2012·Poultry Science·S ChowdhuryA K Ersbøll
Jul 3, 2013·Journal of Microbiological Methods·Ewa PacholewiczBirgitta Duim
Feb 4, 2014·Applied and Environmental Microbiology·Diana SeinigeCorinna Kehrenberg
Dec 3, 2014·International Journal of Food Microbiology·Tomasz SeliwiorstowLieven De Zutter
Mar 21, 2015·Food Microbiology·A DuarteM Uyttendaele
Citations
Apr 12, 2016·Zoonoses and Public Health·G SchalleggerB Stessl
Jun 15, 2016·Journal of Food Protection·Tomasz SeliwiorstowLieven De Zutter
Feb 23, 2018·Poultry Science·Andréa G S A CastroSilvana V Cançado
Jan 17, 2021·Pathogens·Karolien HertogsGeertrui Rasschaert