Comparison of secretory responses as measured by membrane capacitance and by amperometry

Biophysical Journal
M HallerE Neher

Abstract

We have compared capacitance and amperometric measurements in bovine chromaffin cells when secretion was elicited by flash photolysis of caged-calcium or step depolarizations. Total amperometric charge depended linearly on the amount of capacitance increase in both types of experiments. Furthermore, the properties of resolvable amperometric spikes after flashes were comparable to those observed after depolarizations, and their timing was compatible with the rate of capacitance increase. For a more detailed comparison, we used Monte Carlo simulations of multiple amperometric events occurring randomly over the surface of a sphere and summing together, to generate a reference amperometric signal for a given measured capacitance increase. Even after correction for endocytotic processes, the time courses of the integrated experimental records lagged behind the integrated Monte Carlo records by approximately 50 ms in flash and depolarization experiments. This delay was larger by approximately 40 ms than what can be expected from the "pre-foot delay" or the foot duration. Possible sources for the remaining delay could be diffusional barriers like the patch-pipette and the chamber bottom, which are not taken into account in the model. ...Continue Reading

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·E Neher, C F Stevens
Dec 11, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M WightmanO H Viveros
Jun 1, 1991·Journal of Neurochemistry·D J LeszczyszynR M Wightman
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E Neher, A Marty
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·C R ArtalejoH C Palfrey
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·I M RobinsonJ M Fernandez
Sep 1, 1993·The Journal of Physiology·Z Zhou, E Neher
Jul 1, 1993·Pflügers Archiv : European journal of physiology·C HeinemannE Neher
Jun 10, 1993·Nature·G Alvarez de ToledoJ M Fernández
Jan 1, 1995·Journal of Physiology, Paris·A F OberhauserJ M Fernandez
Jun 1, 1996·Current Opinion in Neurobiology·A W Henkel, W Almers
Aug 1, 1996·Biophysical Journal·A F OberhauserJ M Fernandez
Nov 1, 1996·Journal of Neuroscience Methods·P MesslerR Uhl
Mar 3, 1997·The EMBO Journal·Y NinomiyaH Kasai
Dec 31, 1997·The Journal of Cell Biology·C Smith, E Neher

❮ Previous
Next ❯

Citations

Nov 20, 2008·Pflügers Archiv : European journal of physiology·Jovita KaranauskaitePatrik Rorsman
Oct 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Amy B HarkinsAaron P Fox
Jan 16, 2008·The Journal of General Physiology·Zhen Zhang, Meyer B Jackson
Sep 30, 2009·The Journal of General Physiology·Jason J LefkowitzValerie De Crescenzo
Dec 18, 2001·The Journal of Physiology·S A Chan, C Smith
May 10, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chad P Grabner, David Zenisek
Feb 16, 2011·Biophysical Journal·G Th H van KempenR H S Westerink
Sep 26, 2006·Advanced Drug Delivery Reviews·Haruo KasaiNoriko Takahashi
Sep 18, 2001·The Journal of Physiology·M SpassovaT D Parsons
Nov 22, 2000·Neuron·D BrunsR Jahn
Jun 2, 2010·Biophysical Journal·Zhen Zhang, Meyer B Jackson
Dec 26, 2006·Biophysical Journal·Janosch Lichtenberger, Peter Fromherz
Mar 29, 2005·Biophysical Journal·Christian AmatoreLeslie A Sombers
Jun 19, 2001·The Journal of Physiology·T KishimotoH Kasai
Jan 19, 2016·Scientific Reports·Panpan HouJiuping Ding
Sep 4, 2017·Pflügers Archiv : European journal of physiology·Kevin D GillisValentina Carabelli
Aug 8, 2006·Journal of Neurochemistry·Alvaro O ArdilesAna M Cárdenas
Oct 20, 2007·Angewandte Chemie·Albert Schulte, Wolfgang Schuhmann
Jun 10, 2005·Journal of Neurophysiology·Chad P GrabnerAaron P Fox
Feb 10, 2006·The EMBO Journal·Takuya KishimotoHaruo Kasai
Mar 16, 2000·Reviews in the Neurosciences·D Sulzer, E N Pothos
Oct 18, 2012·Physiological Reviews·Haruo KasaiHiroshi Tokumaru
Jan 4, 2008·American Journal of Physiology. Cell Physiology·Haini LiaoDavid Castle
Feb 9, 2017·The Journal of General Physiology·Che-Wei ChangMeyer B Jackson
Feb 20, 2008·The Journal of Biological Chemistry·Patricia NecoEva Alés
May 2, 2008·Chemical Reviews·Graham C R Ellis-Davies
Feb 24, 2007·Biophysical Chemistry·Christian AmatoreYann Verchier
Jul 16, 2008·Chemical Reviews·Christian AmatoreFrédéric Lemaître

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.