Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater

Journal of Water and Health
Raphael Corrêa Medeiros, Luiz Antonio Daniel

Abstract

More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wastewater samples using ColorSeed®. Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries.

References

Jul 1, 1993·The Journal of Applied Bacteriology·G VeseyC R Fricker
Jul 6, 2000·Revista Da Sociedade Brasileira De Medicina Tropical·K M LudwigJ T Ribeiro-Paes
May 8, 2001·Revista Do Instituto De Medicina Tropical De São Paulo·R M FrancoR Cantusio Neto
Sep 13, 2002·The American Journal of Tropical Medicine and Hygiene·Maria Das Graças Cabral PereiraMarco Tulio Antonio García-Zapata
Jan 30, 2003·Water Research·Bing Mu Hsu, Hsuan Hsien Yeh
May 17, 2005·Journal of Microbiological Methods·Randi M McCuin, Jennifer L Clancy
Apr 4, 2007·Journal of Water and Health·Panagiotis KaranisHuw Smith
Jun 5, 2007·Cadernos de saúde pública·Júlio César TeixeiraMauricio L Barreto
Nov 21, 2007·Journal of Water and Health·Helen L RisebroPaul R Hunter
Aug 2, 2008·Cadernos de saúde pública·Sheila Maria Alvim MatosMaurício Lima Barreto
May 19, 2009·Revista Da Sociedade Brasileira De Medicina Tropical·Wheverton Ricardo Correia do NascimentoFrancisca Janaina Soares Rocha
Jul 14, 2010·International Journal of Hygiene and Environmental Health·Judit PlutzerPanagiotis Karanis

❮ Previous
Next ❯

Citations

Nov 18, 2016·Environmental Technology·Priscila Ribeiro Dos Santos, Luiz Antonio Daniel
Aug 31, 2016·Environmental Technology·Priscila Ribeiro Dos Santos, Luiz Antonio Daniel
May 29, 2019·Environmental Technology·Gabriela Laila de Oliveira, Luiz Antonio Daniel
Sep 9, 2020·PLoS Neglected Tropical Diseases·Retina ShresthaBhanu Bhakta Neupane
Jan 22, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Nathalie Dyane Miranda SlompoLuiz Antonio Daniel
Dec 19, 2020·Environmental Technology·Fernanda Queiroz ValdezLuiz Antonio Daniel
Jun 22, 2021·Journal of Water and Health·Natália de Melo Nasser FavaLyda Patricia Sabogal-Paz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.