PMID: 7026671Oct 1, 1981Paper

Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water

The Journal of Hygiene
R W Harvey, T H Price

Abstract

Six hundred and eighty three samples of chicken giblets were examined for salmonellas. Three hundred and forty nine of these were neck and crop specimens and 224 were combined liver and heart samples. Two hundred and ten, in all, contained salmonellas. The technique of examination included pre-enrichment in buffered peptone water at 37 degrees C for 18 h and subculture to three enrichment media: Muller-Kauffmann tetrathionate, selenite F and Rappaport's magnesium chloride malachite green broth. Inocula from buffered peptone water to 10 ml of tetrathionate and selenite were 1 ml in each case. The inoculum from the pre-enrichment medium to 10 ml of Rappaport was 0.005 ml. Tetrathionate and selenite were incubated at 43 degrees C for 48 h. Rappaport's medium was incubated at 37 degrees C for 48 h. Subcultures from all three enrichment broths were made at 24 h and 48 h to brilliant green MacConkey agar. Selective agars were incubated at 37 degrees C for 24 h. The most successful technique for salmonella isolation used Rappaport's medium, which was significantly more efficient than either tetrathionate or selenite. This finding reinforces results obtained using sewage polluted natural water as test material and it is suggested that ...Continue Reading

References

Jun 1, 1977·The Journal of Hygiene·R W HarveyJ Morgan
Dec 1, 1979·The Journal of Hygiene·R W HarveyD H Joynson
Dec 1, 1978·The Journal of Applied Bacteriology·P Y Chau, Y K Leung
Jun 1, 1975·The Journal of Hygiene·R W Harvey, T H Price
Sep 1, 1973·The Journal of Hygiene·R W HarveyM L Hall
Dec 1, 1970·The Journal of Hygiene·P VassiliadisG Politi
Aug 1, 1969·Australian Veterinary Journal·G T Chung, A J Frost
Aug 1, 1956·Journal of Clinical Pathology·N KONFORTIF RAPPAPORT
Jul 1, 1961·Journal of Clinical Pathology·J M DIXON

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Citations

Feb 1, 1983·The Journal of Applied Bacteriology·P Vassiliadis
Jul 1, 1986·The Journal of Applied Bacteriology·M D Patil, N M Parhad
Aug 1, 1987·The Journal of Applied Bacteriology·C R Fricker
Aug 17, 2000·Journal of Applied Microbiology·P R DaviesP J Fedorka-Cray
Sep 8, 2010·Journal of Food Protection·Geoffrey MeadUNKNOWN Salmonella On Raw Poultry Writing Committee

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