Comparison of senescence-related changes between three- and two-dimensional cultured adipose-derived mesenchymal stem cells.

Stem Cell Research & Therapy
Qiliang YinWensen Liu

Abstract

Adipose-derived mesenchymal stem cells (ADMSCs) have attracted widespread interest as cell-based tissue repair systems. To obtain adequate quantities of ADMSCs for therapeutic applications, extensive in vitro expansion is required. However, under current two-dimensional (2D) approaches, ADMSCs rapidly undergo replicative senescence, and cell growth is impeded and stem cell properties are eliminated by mechanisms that are poorly understood. These issues limit the extensive applications of ADMSCs. In this study, we investigated senescence-related changes in mesenchymal stem cells (MSCs) isolated from human adipose tissue in 2D and three-dimensional (3D) cultures. We studied cell growth over a given period (21 days) to determine if modes of culture were associated with ADMSC senescence. ADMSCs were isolated from healthy females by liposuction surgery and then were grown in 2D and 3D cultures. The cell morphology was observed during cell culture. Every other time of culture, senescence-associated β-galactosidase (SA-β-gal) expression, cell viability, proliferation, and differentiation potential of ADMSCs from 2D and 3D cultures were detected. Also, senescence- and stemness-related gene expression, telomere length, telomerase activi...Continue Reading

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Citations

Jan 9, 2021·Stem Cell Research & Therapy·Mesude BicerDarius Widera
Dec 17, 2021·Stem Cells and Development·Xunhui GuoJing Liu

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Methods Mentioned

BETA
Flow cytometry
Assay
fluorescence microscopy

Software Mentioned

GraphPad Prism
Muse Cell Analyzer

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