Comparison of separation modes of high-performance liquid chromatography for the analysis of glycoprotein- and proteoglycan-derived oligosaccharides

Journal of Chromatography. a
M J Davies, E F Hounsell

Abstract

Porous graphitised carbon (PGC) has been explored for high-performance liquid chromatography (HPLC) of mono- and di-saccharides released from proteoglycans and of fluorescently labelled oligosaccharide derivatives for high-sensitivity detection. Sulphated oligosaccharides show good retention and separation behaviour on PGC-HPLC, and compared to anion-exchange or reversed-phase ion-pair chromatography the chromatography is carried out in the absence of salt. Due to their poor retention on PGC-HPLC the analysis of single uronic acids has been optimised with high pH anion-exchange chromatography. Fluorescent labelled derivatives formed by reductive amination of neutral oligosaccharides with 2-aminobenzamide have been chromatographed on PGC-HPLC and by BioGel P4 gel filtration.

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J EdgeR A Dwek
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Citations

Nov 1, 1996·Biomedical Chromatography : BMC·M J Davies, E F Hounsell
Feb 28, 2009·Analytical and Bioanalytical Chemistry·L Renee RuhaakManfred Wuhrer
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Roisin A DoohanNiclas Göran Karlsson
Aug 1, 1997·Current Opinion in Biotechnology·P M Rudd, R A Dwek
Nov 30, 2011·Bioanalysis·Serenus HuaHyun Joo An
Jan 17, 2007·Chemistry : a European Journal·Hideyuki ShimaokaShin-Ichiro Nishimura
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis
Oct 15, 2020·Carbohydrate Polymers·Matěj ŠimekVladimír Havlíček

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