Comparison of serologic techniques for the detection of antibodies against feline coronaviruses

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Annamaria Pratelli

Abstract

The seroprevalence of feline coronavirus (FCoV) antibodies was studied in cats in southern Italy. One hundred twenty sera collected from cats belonging to catteries or community shelters and to households were tested for FCoV type I and II antibodies. The virus neutralization (VN) was performed and compared with indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). Ninety-six sera tested positive for FCoV antibodies by VN and ELISA. Interestingly, ELISA revealed 2 more positive sera than did the VN test and 3 more positive sera than did the IFAT. All results were confirmed by Western blotting. ELISA proved to be more sensitive and detected a seroprevalence of about 82%. Considering the cross-reactivity of FCoV type I and type II, ELISA was able to detect antibodies against both serotypes, allowing the use of the assay as a reference test for sera screening. The high prevalence of antibodies observed indicates that FCoVs are common in southern Italian cat populations.

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Citations

Mar 4, 2009·Journal of Feline Medicine and Surgery·Niels C Pedersen
Nov 2, 2011·The Veterinary Clinics of North America. Small Animal Practice·Yvonne DrechslerPedro Paulo V P Diniz
Jan 12, 2010·Veterinary Microbiology·C GilesT Vanniasinkam
Jun 27, 2014·Journal of Feline Medicine and Surgery·Diane D AddieMarina L Meli
Dec 12, 2012·Journal of Feline Medicine and Surgery·Marina L MeliDiane D Addie
Nov 17, 2019·Viruses·Sandra Felten, Katrin Hartmann
Jun 30, 2009·Journal of Feline Medicine and Surgery·Annamaria PratelliZeki Yilmaz
Sep 27, 2020·Journal of Virological Methods·Morgan PearsonGregg A Dean

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
FCS
electrophoresis

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