Comparison of serum galactomannan antigen detection and competitive polymerase chain reaction for diagnosing invasive aspergillosis

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
S BretagneC Cordonnier

Abstract

To improve the diagnosis of invasive aspergillosis (IA), we retrospectively compared competitive polymerase chain reaction (PCR) and sandwich ELISA for detection of serum galactomannan (GM) antigen. We studied 281 serum samples collected weekly during the period at risk for IA from 41 selected hematology patients. Twenty-two patients had confirmed, probable, or suspected IA, according to clinical and mycologic data. Fifteen of them had positive GM titers (87 samples) and 12 had positive PCRs (20 samples). Nineteen of the 20 PCR-positive samples were also GM-positive. Of the 19 patients without IA (83 samples), one had 3 GM-false-positive samples. Neither test anticipated the initiation of antifungal therapy on the basis of clinical suspicion. Both tests were more likely to be positive before death. This study suggests that PCR on serum samples is not more sensitive than GM detection. However, PCR can improve the specificity of the GM test. Together, these noninvasive tests should improve the diagnosis of IA.

References

Jul 1, 1992·Respiratory Medicine·H Puolijoki, K Liippo

❮ Previous
Next ❯

Citations

Jan 26, 2006·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Patrícia RibeiroAida B Sousa
Nov 15, 2007·Current Infectious Disease Reports·L Joseph Wheat
Dec 26, 2001·Diagnostic Microbiology and Infectious Disease·M KretschmarT Nichterlein
Jan 9, 2004·Diagnostic Microbiology and Infectious Disease·Cornelia Lass-FlörlHermann Dietrich
Oct 29, 2000·International Journal of Antimicrobial Agents·J Bille
Nov 25, 2000·International Journal of Antimicrobial Agents·P Martino, C Girmenia
Jul 20, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Z Erjavec, P E Verweij
Aug 6, 2002·Current Opinion in Microbiology·Jean-Paul Latgé, Richard Calderone
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·P E Verweij, J F Meis
Jan 29, 2000·British Journal of Haematology·E C WilliamsonM R Millar
Dec 11, 2002·Mycoses·O MolingP Mian
Aug 23, 2005·Medical Mycology·D Buchheidt, M Hummel
May 16, 2009·Medical Mycology·Georg MaschmeyerJohn Perfect
Feb 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S J LinS M Teutsch
Jun 5, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J H RexT Wu
Jul 20, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D BuchheidtR Hehlmann
Jan 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Lewis WhiteRosemary A Barnes
Apr 19, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Christopher D PfeifferNasia Safdar
Nov 4, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nikolaos G AlmyroudisBrahm H Segal
Apr 20, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E J Anaissie
Apr 19, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marisa H MiceliElias Anaissie
Sep 29, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M RuhnkeUNKNOWN Infectious Diseases Working Party in Haematology and Oncology of the German Society for Haematology and Oncology
Feb 24, 2001·Transplantation·J FortunUNKNOWN Ramon y Cajal Hospital's Liver Transplant Group
Feb 26, 2004·Antimicrobial Agents and Chemotherapy·Melissa D JohnsonJohn H Rex
Jul 5, 2002·Clinical Microbiology Reviews·Siew Fah Yeo, Brian Wong
Jan 18, 2005·Clinical Microbiology Reviews·Nina Singh, David L Paterson
Feb 22, 2008·Journal of Clinical Microbiology·Monique A S H Mennink-KerstenPaul E Verweij
Sep 24, 2011·Journal of Clinical Microbiology·P Lewis WhiteUNKNOWN European Aspergillus PCR Initiative (EAPCRI)
Feb 10, 2004·Journal of Clinical Microbiology·Svetlana ChallierPatrick Berche
Sep 15, 2004·Journal of Clinical Microbiology·Cornelia Lass-FlörlAndreas Petzer
Oct 7, 2005·Journal of Clinical Microbiology·Laurence MillonStéphane Bretagne
Jul 13, 2000·Journal of Clinical Pathology·E C WilliamsonD W Warnock

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.