Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples.

Scientific Reports
Silvia ValledorJavier Castillo

Abstract

This study aims to validate the current diagnostic method for the clinical detection of gastroenteritis. We analyzed 400 stool samples to detect three of the most common enteropathogens: Salmonella spp., Campylobacter spp., and Yersinia enterocolitica. All specimens were tested with a routine clinical diagnosis algorithm and with five real-time PCR assays. A total of 98 specimens (24.5%) were positive for enteropathogens. We found 24 samples positive for Salmonella enterica, 71 positive for Campylobacter spp., and 4 positive for Yersinia enterocolitica. All evaluated methods exhibited a good performance in identifying Salmonella and Yersinia enterocolitica, being the highest positive percent agreement (PPA) value of 95.8% and 100%, respectively. The clinical algorithm showed the highest PPA value identifying Salmonella, due to the enrichment in selenite broth. However, the evaluated methods showed notable differences in the identification of Campylobacter species, obtaining a wide range of PPA values: 59.2%-100%. The clinical algorithm showed the lowest PPA value since it was only able to detect Campylobacter jejuni and Campylobacter coli species. This study revealed the importance of implementing the real-time PCR technique in...Continue Reading

References

Aug 12, 2003·Journal of Medical Microbiology·S G PathmanathanK L Thong
Oct 26, 2011·Nature Reviews. Gastroenterology & Hepatology·Si Ming Man
May 18, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Alicia B CronquistBarbara E Mahon
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
Feb 26, 2016·Journal of Clinical Microbiology·Marc Roger Couturier
Mar 11, 2016·Journal of Clinical Microbiology·Collette FitzgeraldIrving Nachamkin
Nov 5, 2017·Journal of Microbiological Methods·Ariadna MartínM Ángeles Orellana
Dec 27, 2018·Journal of Microbiological Methods·Maria Dullaert-de BoerAdri G M van der Zanden

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Datasets Mentioned

BETA
CP012196.1
1

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

VIASURE
GraphPad QuickCalcs
SPSS
Chromas

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