Comparison of Shiga-like toxin II expression between two genetically diverse lineages of Escherichia coli O157:H7

Journal of Food Protection
Scot E Dowd, Jason B Williams

Abstract

The existence of two separate lineages of Escherichia coli O157:H7 has previously been reported, and research indicates that one of these lineages (lineage I) might be more pathogenic toward human hosts. We postulated that the lineage more pathogenic expresses higher levels of Shiga toxin 2 (Stx2) than do the nonpathogenic lineage II. A comprehensive set of methodologies were used to investigate the difference in Stx2 protein and mRNA expression between the two lineages. An initial Stx2-specific enzyme-linked immunosorbent assay was conducted, and lineage I overall demonstrated significantly more toxin proteins expressed (P < 0.01). Gene expression analyses all showed significantly higher stx2 gene expression in lineage I (P = 0.02). PCR mapping revealed a possible explanation for decreased amounts of stx2 transcripts in the potentially nonpathogenic lineage II isolates, suggesting that genomic changes have modified the toxin-encoding region of the phage. This study provides additional data to support the existence of two diverse lineages of E. coli O157:H7, one of which may have lower pathogenic potential in relation to human hosts. The PCR described also provides a possible screening tool for E. coli O157 populations to diffe...Continue Reading

References

Sep 1, 1989·Molecular Microbiology·A Donohue-RolfeG T Keusch
Apr 29, 1988·Biochemical and Biophysical Research Communications·T WaddellC Lingwood
Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
Feb 13, 1999·Molecular Microbiology·G FrankelS Knutton
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KimA K Benson
Sep 30, 2000·Applied and Environmental Microbiology·S YaronK R Matthews
Jan 3, 2001·Applied and Environmental Microbiology·H KobayashiK Yamamoto
Jun 12, 2003·Nucleic Acids Research·Jean-Marie RouillardErdogan Gulari
Oct 22, 2004·Emerging Infectious Diseases·Jeffrey T LejeuneSrinand Sreevatsan
Mar 17, 2006·BMC Microbiology·Scot E Dowd, Hiroshi Ishizaki

❮ Previous
Next ❯

Citations

Mar 23, 2011·Applied and Environmental Microbiology·Mark EppingerThomas A Cebula
Jan 25, 2012·FEMS Microbiology Letters·Kjersti HaugumLin Thorstensen Brandal
Dec 2, 2009·Applied and Environmental Microbiology·Yongxiang ZhangVictor P J Gannon
Mar 13, 2009·Foodborne Pathogens and Disease·Brigitte LefebvreFrançois Malouin
Jul 16, 2010·Journal of Food Science·Reuven RasoolyMendel Friedman
Jun 16, 2009·Applied and Environmental Microbiology·Ross M S LoweRanjana Sharma
Jan 13, 2015·Journal of Food Protection·Joseph M BosilevacMohammad Koohmaraie
Sep 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nadejda LupolovaDavid L Gally

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.