Abstract
Constructs intended for bone tissue engineering are influenced by the initial cell seeding procedure. The seeding method should be rapid, convenient, improve cell spatial distribution, and have no negative effects on cellular viability and differentiation. This study aimed to compare the effect of short-run seeding methods (centrifuge and vortex) with a static method on the scaffolds prepared from poly(L-lactide-co-1,5-dioxepan-2-one) by solvent-casting particulate-leaching (SCPL) technique. Human osteoblast-like cells (HOB) were seeded by the three methods described above. The seeding efficiency was determined by attached cell numbers. Cellular proliferation was analyzed by WST-1 and dsDNA assay. Cell distribution was examined by scanning electron (SEM) and fluorescence microscopy. Expression of alkaline phosphatase (ALP), collagen type I (Col I), osteocalcin (OC) and proliferating cell nuclear antigen (PCNA) were determined by real time RT-PCR. Results indicated that centrifuge and vortex increased seeding efficiency and had no negative effects on cellular viability. The data obtained by the fluorescence microscope confirmed the SEM results that the vortex method improved cell distribution through the scaffolds more than the ...Continue Reading
References
Jan 1, 1984·Metabolic Bone Disease & Related Research·J N BeresfordR G Russell
Sep 21, 2001·Clinical Oral Implants Research·K MustafaK Arvidson
May 15, 2002·Biomacromolecules·Maria Ryner, Ann-Christine Albertsson
Jul 13, 2002·Journal of Biomedical Materials Research·K J L BurgG A Johnson
Sep 13, 2002·Biotechnology and Bioengineering·Ayelet DarSmadar Cohen
Dec 3, 2002·Tissue Engineering·Twana DavissonAnthony Ratcliffe
Apr 5, 2003·Biotechnology Progress·Sunil Saini, Timothy M Wick
Sep 11, 2003·Biotechnology and Bioengineering·D WendtI Martin
Feb 14, 2004·Biomaterials·W T GodbeyAnthony Atala
Sep 17, 2004·Biomaterials·Néha DattaAntonios G Mikos
Feb 3, 2005·Tissue Engineering·Alejandro J Almarza, Kyriacos A Athanasiou
May 20, 2005·American Journal of Veterinary Research·Dominique J GriffonRussell Jamison
Sep 13, 2005·Biomacromolecules·Karin OdeliusAnn-Christine Albertsson
Oct 26, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kazunori ShimizuHiroyuki Honda
Nov 22, 2005·Biophysical Journal·Gang ChengKyriacos Zygourakis
Jun 13, 2006·Biomaterials·Lorenzo SolettiDavid A Vorp
Aug 8, 2006·Tissue Engineering·Luis A SolchagaJean F Welter
Jun 26, 2007·Biomaterials·Haiyan LiLeslie Y Yeo
Sep 21, 2007·Tissue Engineering·Jason D RohChristopher K Breuer
Dec 8, 2009·Biomaterials·Paschalia M MountziarisAntonios G Mikos
Citations
Jul 3, 2020·Polymers·Zhe XingMustafa Kamal
Oct 8, 2015·Tissue Engineering. Part C, Methods·Stefano SivolellaBarbara Zavan
Sep 28, 2012·Journal of Biomedical Materials Research. Part a·Zhe XingKamal Mustafa
Feb 19, 2014·Stem Cell Research & Therapy·Torbjorn O PedersenKamal Mustafa
May 28, 2015·Journal of Biomedical Materials Research. Part a·Mohammed A YassinKamal Mustafa
Oct 18, 2016·Biotechnology and Bioengineering·Ines WestphalMatthias Schieker
Apr 19, 2016·Journal of Biomedical Materials Research. Part a·Mohammed A YassinKamal Mustafa
Jun 7, 2021·Biomaterials·Kevin CollonJay R Lieberman
Feb 25, 2014·Biomacromolecules·Yang SunAnn-Christine Albertsson