Comparison of short-run cell seeding methods for poly(L-lactide-co-1,5-dioxepan-2-one) scaffold intended for bone tissue engineering

The International Journal of Artificial Organs
Zhe XingKamal Mustafa

Abstract

Constructs intended for bone tissue engineering are influenced by the initial cell seeding procedure. The seeding method should be rapid, convenient, improve cell spatial distribution, and have no negative effects on cellular viability and differentiation. This study aimed to compare the effect of short-run seeding methods (centrifuge and vortex) with a static method on the scaffolds prepared from poly(L-lactide-co-1,5-dioxepan-2-one) by solvent-casting particulate-leaching (SCPL) technique. Human osteoblast-like cells (HOB) were seeded by the three methods described above. The seeding efficiency was determined by attached cell numbers. Cellular proliferation was analyzed by WST-1 and dsDNA assay. Cell distribution was examined by scanning electron (SEM) and fluorescence microscopy. Expression of alkaline phosphatase (ALP), collagen type I (Col I), osteocalcin (OC) and proliferating cell nuclear antigen (PCNA) were determined by real time RT-PCR. Results indicated that centrifuge and vortex increased seeding efficiency and had no negative effects on cellular viability. The data obtained by the fluorescence microscope confirmed the SEM results that the vortex method improved cell distribution through the scaffolds more than the ...Continue Reading

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Citations

Oct 8, 2015·Tissue Engineering. Part C, Methods·Stefano SivolellaBarbara Zavan
Feb 19, 2014·Stem Cell Research & Therapy·Torbjorn O PedersenKamal Mustafa
May 28, 2015·Journal of Biomedical Materials Research. Part a·Mohammed A YassinKamal Mustafa
Oct 18, 2016·Biotechnology and Bioengineering·Ines WestphalMatthias Schieker
Apr 19, 2016·Journal of Biomedical Materials Research. Part a·Mohammed A YassinKamal Mustafa
Jun 7, 2021·Biomaterials·Kevin CollonJay R Lieberman

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