Comparison of single and double detection points Taylor Dispersion Analysis for monodisperse and polydisperse samples

Journal of Chromatography. a
Joseph Chamieh, Hervé Cottet

Abstract

The purpose of this work was to compare single and double detection points Taylor Dispersion Analysis (TDA) of monodisperse and polydisperse samples for low injected volumes. For that, the hydrodynamic radii of human and bovine serum albumins, a poly(styrene sulfonate) standard and two dendrigraft poly-L-lysines were determined by TDA on a capillary electrophoresis instrument using a modified detection interface allowing the analysis on two detection points. The results were compared to values obtained via a single detection point TDA with adequate corrections for the finite injected volume. The results showed no significant difference between the double and single detection points when Taylor conditions were respected and when the injected volume was less than 1% of the capillary volume till the detection window. The issue of the determination of the peak variance of the Taylorgram in the case of polydisperse sample with non-Gaussian peak is addressed. A criterion is proposed to define the interval of time on which the peak variance is calculated by integration as a function of the signal to noise ratio.

References

Jun 3, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Barbara JachimskaZbigniew Adamczyk
Mar 17, 2012·Journal of Chromatography. a·Joseph ChamiehHervé Cottet

❮ Previous
Next ❯

Citations

Dec 4, 2014·Journal of Chromatography. a·Adam T SuttonPatrice Castignolles
Dec 4, 2015·Biomacromolecules·Joseph ChamiehHervé Cottet
Jul 5, 2015·International Journal of Pharmaceutics·Joseph ChamiehHervé Cottet
Jul 21, 2015·Journal of Chromatography. a·Seyi Latunde-DadaOksana Iryna Leszczyszyn
Jun 18, 2014·Analytical Chemistry·Luca CipellettiHervé Cottet
May 23, 2017·Analytical Chemistry·Phoonthawee SaetearHervé Cottet
Jan 24, 2020·Small·Patricia Taladriz-BlancoSandor Balog
May 18, 2021·Analytical Methods : Advancing Methods and Applications·Meagan R Moser, Christopher A Baker
Dec 18, 2018·Analytical Chemistry·Philipp LemalSandor Balog
Dec 10, 2019·Analytical Chemistry·Patricia Taladriz-BlancoSandor Balog
Jun 28, 2019·Analytical Chemistry·Patricia Taladriz-BlancoSandor Balog

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.