Comparison of Singleplex Specific IgE Detection Immunoassays: ImmunoCAP Phadia 250 and Immulite 2000 3gAllergy

Annals of Laboratory Medicine
Kyung Hee ParkSang Chul Lee

Abstract

The identification of clinically meaningful specific immunoglobulin E (sIgE) is important for the diagnosis and management of allergic diseases. Various in vitro sIgE detection methods are available worldwide. Depending on the number of antigens that can be tested simultaneously, there are two representative methods: singleplex and multiplex. Singleplex sIgE detection is mainly provided by Thermo Fisher (ImmunoCAP) and Siemens (Immulite). This study aimed to compare the diagnostic agreement of two singleplex sIgE detection assays. Sera from 209 Korean patients with allergic disease were used to compare the ImmunoCAP and Immulite assays with respect to the following allergens: inhalant allergens (Dermatophagoides farinae, cat and dog dander, oak, rye grass, mugwort, Alternaria, German cockroach) and food allergens (hen's egg white, cow's milk, wheat, peanut, soybean, and shrimp). Data from 902 paired comparison tests were included for comparisons. Qualitative, semi-quantitative, and quantitative comparisons were performed using statistical analyses. In qualitative comparisons, the positivity and negativity agreements ranged from 75% (wheat, shrimp) to 96% (Alternaria). Class consistency (classes 0-6) was well matched. Spearman's...Continue Reading

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Citations

Oct 31, 2018·Scandinavian Journal of Clinical and Laboratory Investigation·Jinyoung YangEun-Jee Oh
Apr 4, 2018·Annals of Laboratory Medicine·Hyunjin JeonSeon Tae Kim
Nov 12, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Ekaterina PotapovaPaolo Maria Matricardi
May 19, 2020·Allergologia et immunopathologia·F-X TengY-B Cui
Aug 7, 2020·Biosensors & Bioelectronics·Salvador MasÁngel Maquieira
Mar 5, 2020·The World Allergy Organization Journal·Ignacio J AnsoteguiTorsten Zuberbier

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Software Mentioned

Immulite
MedCalc
SPSS

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