Comparison of skeletal and soft tissue pericytes identifies CXCR4+ bone forming mural cells in human tissues.

Bone Research
Jiajia XuAaron W James

Abstract

Human osteogenic progenitors are not precisely defined, being primarily studied as heterogeneous multipotent cell populations and termed mesenchymal stem cells (MSCs). Notably, select human pericytes can develop into bone-forming osteoblasts. Here, we sought to define the differentiation potential of CD146+ human pericytes from skeletal and soft tissue sources, with the underlying goal of defining cell surface markers that typify an osteoblastogenic pericyte. CD146+CD31-CD45- pericytes were derived by fluorescence-activated cell sorting from human periosteum, adipose, or dermal tissue. Periosteal CD146+CD31-CD45- cells retained canonical features of pericytes/MSC. Periosteal pericytes demonstrated a striking tendency to undergo osteoblastogenesis in vitro and skeletogenesis in vivo, while soft tissue pericytes did not readily. Transcriptome analysis revealed higher CXCR4 signaling among periosteal pericytes in comparison to their soft tissue counterparts, and CXCR4 chemical inhibition abrogated ectopic ossification by periosteal pericytes. Conversely, enrichment of CXCR4+ pericytes or stromal cells identified an osteoblastic/non-adipocytic precursor cell. In sum, human skeletal and soft tissue pericytes differ in their basal ab...Continue Reading

References

Jan 11, 2002·The New England Journal of Medicine·Eileen M ShoreFrederick S Kaplan
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Aug 31, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ken KumagaiGeorge F Muschler
May 28, 2008·Experimental Hematology·Antonio SorrentinoMauro Valtieri
Jul 3, 2008·Stem Cells and Development·Jinping XuSunil Kapila
Sep 13, 2008·Cell Stem Cell·Masoud TavazoieFiona Doetsch
Sep 13, 2008·Cell Stem Cell·Mihaela CrisanBruno Péault
Sep 20, 2008·Science·Wei TangJonathan M Graff
Jun 23, 2010·PloS One·Benjamin LeviMichael T Longaker
Jan 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qiqun ZengXiyun Yan
Jun 6, 2012·Journal of Visualized Experiments : JoVE·Aaron W JamesChia Soo
Dec 1, 2012·Stem Cells Translational Medicine·Aaron W JamesChia Soo
Jan 1, 2013·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Claire LugassyRaymond L Barnhill
Feb 16, 2013·Blood·Mirko CorselliBruno Péault
Jun 6, 2013·Biochimica Et Biophysica Acta·Allison J Richard, Jacqueline M Stephens
Nov 6, 2013·Journal of Cellular and Molecular Medicine·Nicolas EspagnollePhilippe Bourin
Nov 6, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Brya G MatthewsIvo Kalajzic
Aug 27, 2014·Stem Cells Translational Medicine·Choon G ChungChia Soo
Sep 23, 2014·Clinical Science·Alexander BirbrairOsvaldo Delbono
Dec 4, 2014·Cell Stem Cell·Rafael KramannBenjamin D Humphreys
Aug 1, 2013·Journal of Biomedical Science and Engineering·Maureen BeedermanTong-Chuan He
Mar 22, 2016·Scientific Reports·T TawonsawatrukA H R W Simpson
Aug 12, 2016·Pharmacology & Therapeutics·Aaron W JamesChia Soo
Sep 30, 2016·Kidney International·Ania StefanskaBruno Péault
Nov 12, 2016·Translational Stroke Research·Wei CaiXiaoming Hu

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Citations

Nov 25, 2020·Tissue Engineering. Part B, Reviews·Elana M MeijerCaroline Cheng
Jan 30, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ginny Ching-Yun HsuAaron W James
Apr 11, 2021·Nature Reviews. Molecular Cell Biology·Alexander Rauch, Susanne Mandrup

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Datasets Mentioned

BETA
GSE128889

Methods Mentioned

BETA
flow cytometry
FACS
PCR
chips
single-cell sequencing
transgenic
fluorescence-activated cell sorting
Assay
electrophoresis
X-ray

Software Mentioned

Seurat
IPA
QIAGEN Ingenuity Pathway Analysis ( IPA )
CTAn
FlowJo
CellRanger
Partek Genomics Suite
QIAGEN
NRecon
GraphPad Prism

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