Comparison of solid-phase immune electron microscopy by use of protein A with direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in stool.

Journal of Clinical Microbiology
L SvenssonC A Pettersson

Abstract

A total of 525 stool specimens collected during 1 year were examined for the presence of rotavirus by direct electron microscopy (EM), enzyme-linked immunosorbent assay (ELISA), and a solid-phase immune electron microscope method (SPIEM) utilizing protein A-coated grids for anchoring of specific viral antisera. Rotavirus was seen in 187 specimens; SPIEM detected 183 (97.8%), whereas direct EM and ELISA detected 161 (86%) and 166 (88.7%), respectively. No false-positive reactions were seen by ELISA. The sensitivity of the methods was evaluated by coded investigation of a dilution series of a positive sample, with a negative fecal specimen as diluent. SPIEM was approximately 30 times more sensitive than direct EM and 10 times more sensitive than ELISA. A study was done to compare the elapsed time for recognition of rotavirus by SPIEM and EM in 25 randomly selected positive specimens. All virus-positive specimens were detected within 2 min by SPIEM, whereas up to 9 min was required for direct EM. SPIEM with protein A is a highly sensitive method, useful for rapid detection of viruses in clinical specimens. Due to the direct visualization of virus particles by electron microscopy, there is no requirement for monospecific antisera f...Continue Reading

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Citations

Jan 1, 1988·Scandinavian Journal of Infectious Diseases·C H Von Bonsdorf, L Svensson
Dec 1, 1987·Journal of Medical Virology·M GrandienI Uhnoo
Mar 1, 1997·Annals of Tropical Paediatrics·F EspinozaL Svensson
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Jun 16, 2018·Journal of Medical Virology·Sumit SharmaLennart Svensson
Apr 20, 1996·The Veterinary Record·K de Verdier Klingenberg, J Esfandiari
Jul 16, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S S Biel, H R Gelderblom
Jun 1, 1988·Journal of Clinical Microbiology·L SvenssonH B Greenberg
Mar 6, 1999·Clinical and Diagnostic Laboratory Immunology·K de Verdier KlingenbergL Svensson
Sep 1, 1984·Journal of Clinical Microbiology·J H HughesV V Hamparian
Jan 1, 1989·Clinical Microbiology Reviews·M L Christensen
May 1, 1984·Journal of Virological Methods·D Katz, Y Straussman
Sep 1, 1985·Journal of Clinical Microbiology·M CherneskyD DeLong
Nov 29, 2001·Journal of Clinical Microbiology·B R EingJ E Kühn

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