Comparison of some different methods for analysis of thyroid autoantibodies: importance of thyroglobulin autoantibodies

Thyroid : Official Journal of the American Thyroid Association
B LindbergS A Ivarsson

Abstract

Blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (RIA) methods for detecting thyroid autoantibodies. Thyroglobulin autoantibodies (Tg-Ab) were analyzed with a hemagglutination kit and a RIA; thyroid peroxidase autoantibodies (TPO-Ab) were measured with a gelagglutination assay and a RIA. The results of the antibody tests were compared with thyroid function tests (triiodothyronine [T3], thyroxine [T4], thyrotropin [TSH]) and with the results of ultrasound of the thyroid in antibody-positive patients. The correlation of antibody levels between the two methods was higher for TPO-Ab than for Tg-Ab. Moderate to high levels of TPO-Ab correlated to elevated TSH levels. Autoimmune thyroiditis (AIT) was found in 6 of the 141 children. The RIA-based thyroglobulin assay was the only test that identified autoantibodies in all 6 cases. In contrast, the hemagglutination kit thyroglobulin assay failed to identify 4 of the 6 AIT cases.

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Citations

Jan 6, 2009·Thyroid : Official Journal of the American Thyroid Association·Margit ZeherPeter Szodoray
Jun 17, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Renato TozzoliNicola Bizzaro
Oct 3, 2002·American Journal of Clinical Dermatology·Daniela ZauliFrancesco B Bianchi
Oct 8, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Carole Spencer, Shireen Fatemi
Apr 10, 2015·The Journal of International Medical Research·Yue-Rong YanNan-Wei Tong
Feb 14, 2008·The Journal of Clinical Endocrinology and Metabolism·Yushu LiWeiping Teng
Nov 3, 2004·Autoimmunity·Anthony P Weetman
Aug 28, 2019·Journal of Cellular Biochemistry·Ruhai YiYueqing Su

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