Comparison of some recent methods for the differentiation of elevated serum amylase and the detection of macroamylasaemia.

Annals of Clinical Biochemistry
A Van DeunW Lissens

Abstract

A pancreatic isoamylase method (Pancreatic Alpha-Amylase EPS, Boehringer) that uses monoclonal antibodies showed almost complete immunoinhibition of salivary (S) amylase activity with only a minor decrease of pancreatic (P) amylase activity. The method displayed good sensitivity and linearity. The correlations of P-amylase activities determined by this technique with a wheat-germ inhibition method and with agarose electrophoresis followed by densitometric scanning were excellent. However, both the wheat-germ and monoclonal inhibition methods failed to detect macroamylasaemia. To recognise macroamylases we used the PEG precipitation method and confirmed the results with agarose electrophoresis. Of 161 serum samples with elevated amylase activities, only four out of five with macroamylasaemia were detected by the PEG precipitation method. No false positives were demonstrated. After PEG precipitation of 28 samples, P-amylase determinations were performed on the supernatants. Again, four out of five with macroamylasaemia were recognised. We consider P-amylase measurement and, when macroamylasaemia is suspected, the combined use of the PEG precipitation method and P-amylase or total amylase determination to be the most practical way...Continue Reading

References

Nov 16, 1985·British Medical Journal·C E Boyle, C G Fraser
Dec 1, 1985·Digestive Diseases and Sciences·W M Steinberg
Apr 1, 1984·Digestive Diseases and Sciences·J C KolarsM D Levitt
Nov 1, 1983·Digestive Diseases and Sciences·M GerstenS Lendvai

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Citations

Aug 1, 1997·Journal of Clinical Pathology·M R HancockS G Ball
May 1, 1997·Scandinavian Journal of Clinical and Laboratory Investigation·G BonettiR Moratti
Sep 13, 1991·Journal of Chromatography·J A Beeley
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·T Moriyama, H Ikeda
Mar 1, 1990·Annals of Clinical Biochemistry·S J Frost
Mar 1, 1991·Annals of Clinical Biochemistry·T E Mifflin, D E Bruns

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