Comparison of statistical methods for identification of Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium from randomly amplified polymorphic DNA patterns

Applied and Environmental Microbiology
G MoschettiEugenio Parente

Abstract

Thermophilic streptococci play an important role in the manufacture of many European cheeses, and a rapid and reliable method for their identification is needed. Randomly amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) with two different primers coupled to hierarchical cluster analysis has proven to be a powerful tool for the classification and typing of Streptococcus thermophilus, Enterococcus faecium, and Enterococcus faecalis (G. Moschetti, G. Blaiotta, M. Aponte, P. Catzeddu, F. Villani, P. Deiana, and S. Coppola, J. Appl. Microbiol. 85:25-36, 1998). In order to develop a fast and inexpensive method for the identification of thermophilic streptococci, RAPD-PCR patterns were generated with a single primer (XD9), and the results were analyzed using artificial neural networks (Multilayer Perceptron, Radial Basis Function network, and Bayesian network) and multivariate statistical techniques (cluster analysis, linear discriminant analysis, and classification trees). Cluster analysis allowed the identification of S. thermophilus but not of enterococci. A Bayesian network proved to be more effective than a Multilayer Perceptron or a Radial Basis Function network for the identification of S. thermophilus, E. faecium, and E. faecal...Continue Reading

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Citations

Jan 27, 2006·Microbial Ecology·Ian Hewson, Jed A Fuhrman
Jan 5, 2002·Applied and Environmental Microbiology·Hidetoshi UrakawaDavid A Stahl
May 7, 2003·Applied and Environmental Microbiology·Hidetoshi UrakawaDavid A Stahl
Jun 14, 2013·The Journal of General and Applied Microbiology·Ayman Al-MaririNagim-Eldin Sharabi
Jul 19, 2007·Genetika·S G BotinaV V Sukhodolets
Feb 6, 2008·Journal of Basic Microbiology·Gustavo Pelicioli RiboldiJeverson Frazzon

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