Comparison of stool collection and storage on Whatman FTA Elute cards versus frozen stool for enteropathogen detection using the TaqMan Array Card PCR assay

PloS One
Tahaniyat LalaniMark S Riddle

Abstract

The use of Polymerase Chain Reaction (PCR) assays for pathogen detection in travelers' diarrhea (TD) field studies is limited by the on-site processing and storage requirements for fecal specimens. The objectives of this investigation were to i) characterize the pathogen distribution in deployed military personnel with TD using the TaqMan® Array Card PCR (TAC) on frozen stool and diarrheal smears on Whatman FTA Elute cards (FTA cards), and to ii) compare TAC detection of enteropathogen targets using smeared FTA cards and frozen stool, using TAC on frozen stool as the 'reference standard'. Stool samples, obtained from active duty personnel with acute TD enrolled in a field trial, were smeared onto FTA cards and stored at room temperature. A corresponding aliquot of stool was frozen in a cryovial. FTA cards and frozen stool samples were tested at a central lab, using a customized TAC for detection of TD pathogens. 187 paired frozen stool samples and smeared FTA cards were stored for a median of 712 days (IQR 396-750) before testing. Overall detection rates were 78.6% for frozen stool and 73.2% for FTA cards. Diarrheagenic Escherichia coli were the most common bacteria identified. Using the TAC results on frozen stool as the refer...Continue Reading

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Citations

Aug 11, 2020·Current Opinion in Infectious Diseases·Kelsey J Jesser, Karen Levy
Nov 30, 2019·Military Medicine·Tahaniyat LalaniDavid R Tribble
Sep 15, 2019·Journal of Virological Methods·Theresa CromeansJan Vinjé
Oct 17, 2020·Avian Pathology : Journal of the W.V.P.A·Suwarak WannaratanaSomsak Pakpinyo
Mar 24, 2021·International Journal of Legal Medicine·Anqi ChenChengtao Li
Aug 12, 2021·Clinical Microbiology Reviews·Madeleine J RogersCatherine A Gordon

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS

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