PMID: 8947593Nov 1, 1996Paper

Comparison of surface properties of human IgA, IgE, IgG and IgM antibodies with identical and different specificities

Scandinavian Journal of Immunology
C WingrenU B Hansson

Abstract

In this paper, the authors report the use of liquid-liquid partition chromatography (LLPC) in an aqueous polyethylene glycol (PEG)/dextran two-phase system to compare the surface properties (partition properties) of human antibodies and fragments thereof. The surface properties of all the monoclonal antibodies of different classes and subclasses investigated were within the same broad range as that observed for the polyclonal antibodies and no relationship was found between the exposed surfaces of the immunoglobulins (Ig) and their heavy chain isotype. Moreover, Fc fragments from various IgG1, 2 and 4 myeloma proteins were found to exhibit similar surface properties. Employing chimeric antibodies with identical variable regions the authors found that intact IgG1, 2 and 4 displayed identical surface properties, while the corresponding IgA1, IgA2, IgG3, IgE and IgM antibodies differed both from each other and from the IgGs. The surface properties of chimeric IgG3 could be made similar to those of the IgG1, 2 and 4 chimers by partially reducing the length of the hinge section, but new differences in surface properties appeared when their hinges were of similar length. Thus, LLPC can be used to detect differences or similarities in...Continue Reading

Citations

May 23, 2002·Journal of the American Psychoanalytic Association·Robert S Wallerstein
Sep 30, 2010·Biotechnology and Applied Biochemistry·Sanchayita GhoseMartin Allen
Apr 9, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·E MelanderS Mölstad
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gary R McLeanArturo Casadevall
Jun 20, 1997·Biochimica Et Biophysica Acta·U B HanssonU Alkner

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