Comparison of surface proteomes of enterotoxigenic (ETEC) and commensal Escherichia coli strains

Journal of Microbiological Methods
Ulf SommerChristian Morsczeck

Abstract

Pathogenesis of enterotoxigenic Escherichia coli (ETEC) infections involves colonization of the small intestine mediated by cell-surface fimbriae (CS) or colonization fimbriae antigens (CFA). However, protection against reinfection of ETEC is also conferred by somatic antigens rather than by virulence factors. To discover ETEC specific somatic antigens, the surface proteome of the ETEC H10406 strain was compared with that of non-pathogenic E. coli K12 strains. In this study, we were using stable isotope labelling with amino acids in cell culture (SILAC) technology for the labelling and relative quantification of surface proteins in order to identify polypeptides that are specifically present on ETEC strains. Outer membrane proteins were isolated, separated by gel electrophoresis, and identified by mass spectrometry. Twenty-three differentially expressed cell-surface polypeptides of ETEC were identified and evaluated by bioinformatics for protein vaccine candidates. The combination of being surface-exposed and present differentially makes these polypeptides highly suitable as targets for antibodies and thus for use in passive or active immunisation/vaccination.

References

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Citations

Jun 21, 2011·Omics : a Journal of Integrative Biology·Fabio BagnoliRino Rappuoli
Apr 30, 2013·Applied Microbiology and Biotechnology·Laurence Van Oudenhove, Bart Devreese
Aug 30, 2014·FEMS Microbiology Reviews·Joseph J GillespieAbdu F Azad
Jul 10, 2012·Comparative Immunology, Microbiology and Infectious Diseases·Júlia M NaraRoxane M F Piazza
Jul 6, 2015·Journal of Microbiological Methods·Anders BoysenJakob Møller-Jensen
Jan 10, 2012·Journal of Mass Spectrometry : JMS·Richard R DrakeSteven K Drake
Apr 28, 2012·Journal of Microbiological Methods·Yulanda M WilliamsonJohn R Barr
Sep 13, 2016·Protein Science : a Publication of the Protein Society·Cristiane S de SouzaRoxane M F Piazza
Sep 15, 2019·Journal of Separation Science·Yanan LiMingliang Ye

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