Comparison of Survival and Osteogenic Ability of Human Mesenchymal Stem Cells in Orthotopic and Ectopic Sites in Mice

Tissue Engineering. Part a
Mathieu ManasseroHervé Petite

Abstract

Tissue constructs containing mesenchymal stem cells (MSCs) are appealing strategies for repairing large segmental bone defects, but they do not allow consistent bone healing and early cell death was identified as a cause of failure. However, little is known about cell survival in the clinical microenvironment encountered during bone healing process. Osteoconductive coral scaffold with or without luciferase-labeled human MSCs were implanted either in a critical segmental femoral bone defect stabilized by plate or subcutaneously in 44 mice. Cell survival was evaluated by serial bioluminescence imaging (BLI) and osteogenic capabilities by histology and microcomputed tomography. Comparisons between groups were performed with two-way analysis of variance test. Twenty mice were sacrificed 2 weeks after surgery for short-term evaluation and 24 mice at 10 weeks for long-term evaluation. BLI provided evidence of fast and continuous cell death: 85% decrease of the BLI signal over the first 2 weeks in both locations; in fact, less than 2% of the initial cell number was present in all constructs analyzed 4 weeks postimplantation and less than 1% of the initial cell number by 8 weeks postimplantation. By 2 weeks postimplantation, the amount...Continue Reading

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Citations

Oct 30, 2016·Orthopaedics & Traumatology, Surgery & Research : OTSR·E RaposioF Calderazzi
Sep 7, 2017·Journal of Tissue Engineering and Regenerative Medicine·Adrien MoyaDelphine Logeart-Avramoglou
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Jun 21, 2018·Bone & Joint Research·E Ferreira, R M Porter
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Oct 23, 2020·Biotechnology Journal·Sean D MadsenKim C O'Connor
Jun 3, 2021·International Journal of Molecular Sciences·Agnese GugliandoloFrancesca Diomede

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Methods Mentioned

BETA
flow cytometry
bioluminescence imaging

Software Mentioned

GraphPad Prism
GraphPad

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