Comparison of Synthetic Media Designed for Expansion of Adipose-Derived Mesenchymal Stromal Cells

Biomedicines
Michelle LenschDavid Harris

Abstract

Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible. Multiple synthetic, xeno-free media have been developed to combat these limitations of animal-derived growth serum and have the potential to be used in ex vivo MSC expansion for clinical use. The goal of this study was to determine if xeno-free media are adequate to significantly and efficiently expand MSCs derived from adipose tissue. MSCs were cultured in both standard FBS-containing as well as xeno-free media. The media were compared for cell yield, viability, and phenotypic expression via flow cytometry and directed differentiation. The xeno-free media that were tested were StemMACS MSC Expansion Media (Miltenyi Bio...Continue Reading

References

Feb 21, 2006·Trends in Biotechnology·John K FraserMarc H Hedrick
Dec 3, 2009·Tissue Engineering. Part a·Eugene Yong-Shun SeeJames Cho Hong Goh
Mar 21, 2012·Pediatric Research·German A NorambuenaChristian Jorgensen
Feb 4, 2016·Stem Cells Translational Medicine·Simone RiisTrine Fink
Nov 2, 2016·Frontiers in Cell and Developmental Biology·Carla DesselsMichael S Pepper

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Citations

Jun 13, 2020·Frontiers in Pharmacology·Darshana S CherianTracy S P Heng

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Methods Mentioned

BETA
bone marrow aspiration
flow cytometry
light microscopy

Software Mentioned

StemPro
FACS DIVA

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