Comparison of T24H-his, GST-T24H and GST-Ts8B2 recombinant antigens in western blot, ELISA and multiplex bead-based assay for diagnosis of neurocysticercosis

Parasites & Vectors
Ana Hernández-GonzálezSukwan Handali

Abstract

Currently, the reference standard assay for the serodiagnosis of neurocysticercosis (NCC) is the lentil lectin-bound glycoproteins/enzyme-linked immunoelectrotransfer blot (LLGP-EITB). The main disadvantage of this technique is the complexity of obtaining and purifying the LLGP extract. This could be solved by replacement with highly specific recombinant antigens from Taenia solium. Based on previous studies, we selected and produced the recombinant Ts8B2 and T24H proteins and applied them to three diagnostic techniques: western blot (WB), enzyme-linked immunosorbent assay (ELISA) and the multiplex bead-based assay (MBA). The Ts8B2 and T24H cDNA sequences were expressed in a prokaryotic system and the corresponding expression products purified; three recombinant proteins were further characterized: T24H-his, GST-T24H and GST-Ts8B2. The proteins on WB, ELISA and MBA were tested against 149 sera from patients with NCC confirmed by brain imaging, 40 sera from patients with other parasitic diseases, and 131 sera from US. individuals without evidence of neurocysticercosis (clinical/serological/brain imaging). The sensitivity and specificity of each antigen by WB were calculated by counting the number of true positive, false positive...Continue Reading

References

Oct 1, 1996·Journal of the Neurological Sciences·O H Del BruttoP M Schantz
Aug 2, 2001·Neurology·O H Del BruttoH H García
Dec 12, 2003·Molecular and Biochemical Parasitology·Kathy HancockVictor C W Tsang
Dec 20, 2005·Parasitology International·Wulamu MamutiAkira Ito
Feb 2, 2006·Journal of Immunological Methods·Sonela A SchlottmannMark T Esser
Mar 17, 2006·Molecular and Biochemical Parasitology·Kathy HancockVictor C W Tsang
Aug 29, 2009·Molecular and Biochemical Parasitology·Elizabeth FerrerTeresa Gárate
Nov 13, 2009·Clinical and Vaccine Immunology : CVI·Sukwan HandaliPatricia P Wilkins
Jan 20, 2010·Trends in Parasitology·Nynke Deckers, Pierre Dorny
Apr 5, 2011·The American Journal of Tropical Medicine and Hygiene·Yeuk-Mui LeeVictor C W Tsang
Mar 19, 2013·PLoS Neglected Tropical Diseases·Agnès FleuryR Michael Evans Parkhouse
Apr 30, 2013·Epilepsia·Gagandeep SinghJosemir W Sander
Aug 8, 2013·PloS One·Lorenzo ZammarchiUNKNOWN COHEMI Project Study Group
Feb 21, 2014·Journal of Clinical Microbiology·John NohPatricia P Wilkins
Jul 6, 2014·PLoS Neglected Tropical Diseases·Paul L A M CorstjensUNKNOWN Cysticercosis Working Group in Peru
May 20, 2015·Emerging Infectious Diseases·Seth E O'Neal, Robert H Flecker
Jun 16, 2016·The New England Journal of Medicine·Hector H GarciaUNKNOWN Cysticercosis Working Group in Peru

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Citations

Apr 13, 2018·PLoS Neglected Tropical Diseases·Rafaella P M Guimarães-PeixotoAbelardo Silva-Júnior
Jun 8, 2018·Journal of Clinical Microbiology·Hector H GarciaUNKNOWN Cysticercosis Working Group in Peru
Jun 3, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Gabriela B da SilvaJulia M Costa-Cruz
Mar 17, 2019·Parasitology Research·Vanessa da Silva RibeiroJulia Maria Costa-Cruz
Feb 3, 2020·Parasitology Research·Jesica A HerrickRon Hershow

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Datasets Mentioned

BETA
AJ508918.1
AY211879.1
BF643023

Methods Mentioned

BETA
the
ELISA

Software Mentioned

BioPlex manager
SoftMax Pro
SPSS

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