Comparison of testing methods for detection of decreased linezolid susceptibility due to G2576T mutation of the 23S rRNA gene in Enterococcus faecium and Enterococcus faecalis

Journal of Clinical Microbiology
Chao QiJohn R Warren

Abstract

E-test, Vitek 2, MicroScan, agar dilution, and disk diffusion were compared for detection of decreased linezolid susceptibility due to 23S rRNA gene G2576T mutation among 32 clinical Enterococcus strains initially reported as intermediate or resistant by E-test alone or Vitek 2 confirmed by E-test. Agar and broth dilution methods were in concordance with PCR detection of the mutation, and disk diffusion was somewhat less sensitive but equally specific.

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Citations

Jun 5, 2013·Antimicrobial Agents and Chemotherapy·Milena McLaughlinMarc H Scheetz
Jul 20, 2007·Journal of Clinical Microbiology·Fred C TenoverJohn E McGowan
Jan 25, 2008·Emerging Infectious Diseases·Marion A KainerWilliam Schaffner
Nov 3, 2012·Environmental Microbiology·Veronika OravcovaIvan Literak
Aug 8, 2013·Environmental Microbiology·Veronika OravcovaIvan Literak
Aug 26, 2006·Diagnostic Microbiology and Infectious Disease·Marc H ScheetzTeresa R Zembower
Mar 5, 2016·Journal of Clinical Microbiology·Christopher D DoernCarey-Ann D Burnham
Apr 9, 2009·Expert Opinion on Pharmacotherapy·Jiun-Ling Wang, Po-Ren Hsueh
May 26, 2020·The Journal of Antimicrobial Chemotherapy·Loren DejoiesVincent Cattoir
Feb 8, 2007·The Annals of Pharmacotherapy·Fotinie Ntziora, Matthew E Falagas
Jun 3, 2021·Clinical Microbiology Reviews·Stefan SchwarzYang Wang

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