Comparison of tetrahydrofuran and ethyl acetate as extraction solvents for urinary organic acid analysis

Journal of Inherited Metabolic Disease
Gy WittmannS Túri

Abstract

The analysis of urinary organic acids is crucial for the diagnosis of many inborn errors of metabolism. A vital part of the analytical process is the extraction procedure. The sensitivity and linearity of the analysis of 26 diagnostically important urinary metabolites with tetrahydrofuran (THF) and ethyl acetate (EtOAc) as extraction solvents were determined by gas chromatography-mass spectrometry. Good linearity (r (2) > 0.90) was observed for all of the compounds in the investigated concentration range (290-900 mumol/L) for both solvents. For less polar compounds, THF extraction yielded lower or similar sensitivities as compared with EtOAc (sensitivity ratio: 0.6-1.3). For more polar compounds, however, much higher sensitivities were observed when THF was used (sensitivity ratio: 1.8-17.2). Our results provide information concerning the use of THF for the sensitive quantitative analysis of polar urinary metabolites which are difficult to quantify using EtOAc.

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Citations

Sep 1, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·N Pérez VasquezF Moussa
Mar 16, 2018·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Renata C GallagherUNKNOWN ACMG Biochemical Genetics Subcommittee of the Laboratory Quality Assurance Committee
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laurynas JarukasLiudas Ivanauskas
May 12, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Qichen ZhangJingyuan Xiong

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