PMID: 8595830Sep 1, 1995Paper

Comparison of tetrazolium colorimetric and 51Cr release assays for cytotoxicity determination of dental biomaterials

Dental Materials : Official Publication of the Academy of Dental Materials
T A BeanD M Yourtee

Abstract

The purpose of this study was to compare a methylthiazole tetrazolium (MTT) dye colorimetric method with the standard 51Cr assay as methods of assessing cytotoxicity of dental materials. Two MTT-based colorimetric formats, test tube and 96-well microplate methods, were compared to the 51Cr release assay. A series of eight dental materials were evaluated. Cytotoxicity profiles were determined for each test material. A TC50 value (Toxic Concentration required to kill 50% of the cells) was determined for each biomaterial, and these results were used to make statistical comparisons between the methods. The three methods were statistically correlated (p<0.005) by comparison of the eight samples tested. That is, the same rank in toxicity was given by the two tetrazolium sample formats and the 51Cr method. The MTT assay was found to have several advantages in comparison to the current standard 51Cr release assay. Optimized in the 96-well format, complete dose response curves and greater sample comparisons can be made rapidly, making the MTT method more economical in time and cost. Furthermore, the MTT method is based on intracellular biochemical changes, measuring cell viability rather than cell morbidity, and has lower detectable lim...Continue Reading

References

Jan 1, 1992·Dental Materials : Official Publication of the Academy of Dental Materials·J C WatahaC T Hanks
Nov 1, 1991·Journal of Dental Research·C T HanksR G Craig
Mar 1, 1973·Oral Surgery, Oral Medicine, and Oral Pathology·L Spangberg
Apr 1, 1993·Biomaterials·G CiapettiA Pizzoferrato

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Citations

Mar 29, 2002·Journal of Biomedical Materials Research·Chern-Chin ChenShun-Te Huang
May 13, 2006·Archives of Toxicology·Franz-Xaver ReichlReinhard Hickel
Sep 17, 2005·Journal of Materials Science. Materials in Medicine·A P MarquesR L Reis
Jun 23, 2000·Dental Materials : Official Publication of the Academy of Dental Materials·E L KostoryzD M Yourtee
Dec 5, 2012·Dental Materials Journal·Ayse AtayPelin Guneri
Nov 3, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F H AbdallaM B Moretto
Feb 12, 2004·International Endodontic Journal·A NalçaciS Yilmaz
Nov 6, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kutlu Gokhan OzdemirSukran Yilmaz
Aug 21, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Bilge Turhan BalSükran Yilmaz
Aug 6, 1999·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·A T TangJ Ekstrand
Sep 7, 2016·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Canan AkayHandan Sevim
Mar 16, 2000·Journal of Biomaterials Science. Polymer Edition·E L KostoryzD M Yourtee

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