Comparison of TGF-beta/BMP pathways signaled by demineralized bone powder and BMP-2 in human dermal fibroblasts

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Shuanhu ZhouKaren E Yates

Abstract

Demineralized bone induces chondrogenic differentiation of human dermal fibroblasts in vitro. Analyses of signaling gene expression showed that DBP and BMP-2 regulate common and distinct pathways. Although BMP-2 was originally isolated as a putative active factor in DBP, rhBMP-2 and DBP do not affect all the same genes or in the same ways. Demineralized bone powder (DBP) induces chondrogenic differentiation of human dermal fibroblasts (hDFs) in 3D culture, but the initiating mechanisms have not been identified. We tested the hypotheses that DBP would affect expression of signaling genes and that DBP's effects would differ from the effects of bone morphogenetic proteins (BMPs). A chondroinduction model was used in which hDFs were cultured with and without DBP in a porous collagen sponge. BMP-2 was delivered in a square of absorbable collagen felt inserted into a collagen sponge. Total RNA was isolated after 3 days of culture, a time that precedes expression of the chondrocyte phenotype. Gene expression was evaluated with two targeted macroarray screens. Effects of DBP and rhBMP-2 were compared by macroarray, RT-PCR, and Northern hybridization analysis of selected genes in the transforming growth factor (TGF)-beta/BMP signaling p...Continue Reading

References

Nov 1, 1985·Clinical Orthopaedics and Related Research·N Muthukumaran, A H Reddi
May 13, 1983·Science·M R UristG A Finerman
Mar 1, 1996·Clinical Orthopaedics and Related Research·J Glowacki
Jun 8, 1996·Annals of the New York Academy of Sciences·V RosenK Lyons
Mar 26, 1998·Biochemical and Biophysical Research Communications·M TakaseM Kawabata
Mar 3, 1999·American Journal of Human Genetics·C J Ring, K W Cho
Jul 3, 1999·The Journal of Biological Chemistry·A HollnagelA Nordheim
Jul 23, 1999·Clinical Orthopaedics and Related Research·R K RosenthalJ Glowacki
Sep 3, 1999·The Orthopedic Clinics of North America·M P BostromT A Einhorn
Feb 5, 2000·Cell·J L Wrana
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J Massagué
Apr 17, 2001·Experimental Cell Research·K E YatesJ Glowacki
Apr 25, 2001·Journal of Cellular Physiology·K MiyazonoH Inoue
Apr 26, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·I K MoutsatsosD Gazit
Jun 8, 2001·Biochemical and Biophysical Research Communications·I SekiyaD J Prockop
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Oct 18, 2001·Clinical Orthopaedics and Related Research·S W O'Driscoll, J S Fitzsimmons
Oct 31, 2001·Differentiation; Research in Biological Diversity·A L CarlbergD J Hall
Nov 6, 2001·Critical Reviews in Eukaryotic Gene Expression·A Hoffmann, G Gross
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R M LocklinS Khosla
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ichiro SekiyaDarwin J Prockop
Apr 9, 2002·Clinical Orthopaedics and Related Research·Hisashi IwataThomas W Bauer
Apr 24, 2002·Developmental Cell·Gerard Karsenty, Erwin F Wagner
Jun 1, 2002·Science·Liliana Attisano, Jeffrey L Wrana
Oct 23, 2002·Molecular and Cellular Biology·Jennifer J WestendorfXiaodong Li
Oct 24, 2002·Journal of Spinal Disorders & Techniques·J Kenneth BurkusThomas A Zdeblick
Dec 10, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Diana S de JongWilma T Steegenga
May 8, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Paola BellostaClaudio Basilico
May 16, 2003·The Journal of Biological Chemistry·Kristina LöhrMatthias Dobbelstein
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Aug 20, 2003·The Journal of Bone and Joint Surgery. American Volume·David L DiefenderferPhoebe S Leboy
Sep 4, 2003·Connective Tissue Research·David L DiefenderferPhoebe S Leboy
Sep 25, 2003·Connective Tissue Research·Karen E Yates, Julie Glowacki
Jan 28, 2004·Cells, Tissues, Organs·Anna M OsyczkaPhoebe S Leboy

❮ Previous
Next ❯

Citations

Jan 1, 2009·Critical Reviews in Biomedical Engineering·Lijie ZhangKyriacos A Athanasiou
Jul 13, 2005·DNA and Cell Biology·Karen E YatesRichard G Reish
May 12, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shuanhu ZhouMeryl S LeBoff
Dec 15, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shuanhu ZhouJulie Glowacki
Aug 28, 2009·The Journal of Investigative Dermatology·Michael Y FessingVladimir A Botchkarev
Jul 26, 2005·Nature Medicine·Takako KogaHiroshi Takayanagi
Oct 18, 2007·Biopolymers·Julie Glowacki, Shuichi Mizuno
Apr 12, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marcus JägerRüdiger Krauspe
Jan 27, 2009·The Journal of Craniofacial Surgery·Julie GlowackiShuichi Mizuno
Sep 25, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Akikazu IshiharaAlicia L Bertone
Oct 2, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Julie HensJohn Wysolmerski
Jul 19, 2005·Orthodontics & Craniofacial Research·J GlowackiS Mizuno
Jun 8, 2007·The Journal of Biological Chemistry·Keertik FulzeleThomas L Clemens
Feb 9, 2017·Stem Cells Translational Medicine·Tingliang WangLian Zhu

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.