PMID: 42219Nov 1, 1979

Comparison of the activity and properties of 5'-nucleotidase in the homogenates and plasma membranes of the normal liver, hepatomas and of the liver in mice with inoculated hepatomas of varying degrees of malignancy

Voprosy medit︠s︡inskoĭ khimii
S Ia Davydova

Abstract

Activity of 5'-nucleotidase was significantly lower in plasmatic membranes of highly malignant hepatoma 22 as compared with the activity found in normal liver tissue. The optimal activity of the enzyme from hepatoma 22 was found at pH 8.5 with AMP as a substrate. Decrease of pH value from 8.5 to 7.4 did not affect the enzymatic activity in homogenates and plasmatic membranes in the normal liver tissue. In all the experiments activity of 5'-nucleotidase was lower towards CMP as compared with AMP. The additive effect of the both substrates was observed only in experiments with hepatoma 22.

Related Concepts

Cyclic AMP, (R)-Isomer
Phosphaden
Plasma Membrane
Enzyme Activation
Hydrogen-Ion Concentration
Liver
Hepatoma, Novikoff
Mice, Inbred C3H
Neoplasm Transplantation
Nucleotidases

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