Comparison of the AdvanSure human papillomavirus screening real-time PCR, the Abbott RealTime High Risk human papillomavirus test, and the Hybrid Capture human papillomavirus DNA test for the detection of human papillomavirus.

Annals of Laboratory Medicine
Yusun Hwang, Miae Lee

Abstract

We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2). All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays. Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as...Continue Reading

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Citations

Sep 18, 2012·Journal of Virological Methods·Jonghyeon ChoiHyon-Suk Kim
Dec 9, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mario PoljakEda Vrtačnik Bokal
Nov 19, 2015·Applied Immunohistochemistry & Molecular Morphology : AIMM·Michel P BihlSylvia Hoeller
Feb 7, 2017·Expert Reviews in Molecular Medicine·D TsakogiannisP Markoulatos

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Methods Mentioned

BETA
PCR
Genotyping

Software Mentioned

BLAST
AdvanSure

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