Comparison of the age-related porcine endogenous retrovirus (PERV)expression using duplex RT-PCR.

Journal of Veterinary Science
Hyoung Joon MoonBong Kyun Park

Abstract

Porcine endogenous retroviruses (PERVs) are members of family Retroviridae, genus Gamma retrovirus, and transmitted by both horizontally and vertically like other endogenous retroviruses (ERVs). PERV was initially described in the 1970s having inserted its gene in the host genome of different pig breeds, and three classes, PERV-A, PERV-B, and PERV-C are known. The therapeutic use of living cells, tissues, and organs from animals called xenotransplantation might relieve the limited supply of allografts in the treatment of organ dysfunction. Because of ethical considerations, compatible organ sizes, and physiology, the pig has been regarded as an alternative source for xenotransplantation. Sensitive duplex reverse transcription-polymerase chain reaction protocols for simultaneously detecting PERV gag mRNA and porcine glyceraldehydes 3-phosphate dehydrogenase mRNA in one tube was established. To compare the age-related PERV expression patterns of the lung, liver, spleen, kidney, heart, and pancreas in commercial pigs, 20 pigs from four age groups (5 heads each in 10 days-, 40 days-, 70 days-, and 110 days-old, respectively) were used in this study. The expression patterns of PERV were statistically different among age groups in lu...Continue Reading

References

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Citations

Feb 11, 2011·Journal of Virology·Eva DörrschuckRalf R Tönjes
Mar 13, 2014·Reproductive Biology·Joanna Gola, Urszula Mazurek
May 16, 2014·Viruses·Magdalena C KimsaUrszula Mazurek
Jun 5, 2010·Xenotransplantation·Mårten K J Schneider, Jörg D Seebach
Jul 8, 2014·Laboratory animal research·Seongsoo HwangSeoki Im
Feb 7, 2012·The International Journal of Artificial Organs·Aleksandra Cyganek-NiemiecUrszula Mazurek

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Methods Mentioned

BETA
xenograft
PCR
electrophoresis

Software Mentioned

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