Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm

Antimicrobial Agents and Chemotherapy
Jaeeun KimJeyong Yoon

Abstract

The systematic understanding of how various antimicrobial agents are involved in controlling biofilms is essential in order to establish an effective strategy for biofilm control, since many antimicrobial agents are effective against planktonic cells but are ineffective when they are used against the same bacteria growing in a biofilm state. Three different antimicrobial agents (chlorine, silver, and tobramycin) and three different methods for the measurement of membrane integrity (plate counts, the measurement of respiratory activity with 5-cyano-2,3-ditolyl tetrazolium chloride [CTC] staining, and BacLight Live/Dead staining) were used along with confocal laser scanning microscopy (CLSM) and epifluorescence microscopy to examine the activities of the antimicrobials on biofilms in a comparative way. The three methods of determining the activities of the antimicrobials gave very different results for each antimicrobial agent. Among the three antimicrobials, tobramycin appeared to be the most effective in reducing the respiratory activity of biofilm cells, based upon CTC staining. In contrast, tobramycin-treated biofilm cells maintained their membrane integrity better than chlorine- or silver-treated ones, as evidenced by imagin...Continue Reading

References

Jun 1, 1974·Canadian Journal of Microbiology·P D Bragg, D J Rainnie
Dec 1, 1994·Applied and Environmental Microbiology·D De BeerP S Stewart
Dec 30, 1998·The Journal of Hospital Infection·J M SchierholzG Pulverer
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·A BroounK Lewis
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·K Lewis
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Sep 15, 2001·Journal of Applied Microbiology·P S StewartW M Rees
Jan 25, 2002·Journal of Bacteriology·Karin SauerDavid G Davies
Jul 18, 2003·Journal of Bacteriology·Jeremy S WebbStaffan Kjelleberg
Nov 19, 2003·Microbes and Infection·Eliana Drenkard
Nov 25, 2003·Nature·Thien-Fah MahGeorge A O'Toole
Dec 2, 2004·American Journal of Infection Control·Mark E RuppPaul D Fey
Jan 11, 2005·Trends in Microbiology·C A FuxP Stoodley
May 27, 2005·Antimicrobial Agents and Chemotherapy·Kimberly K JeffersonGerald B Pier
Jul 2, 2005·Medical Engineering & Physics·E L Lawrence, I G Turner
Aug 1, 2006·American Journal of Infection Control·Debaroti M BorschelSanjay Saint

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Citations

Jun 15, 2011·Journal of Materials Science. Materials in Medicine·Monika Astasov-FrauenhofferTuomas Waltimo
Feb 1, 2011·Biofouling·Soojin ShimJeyong Yoon
Nov 13, 2008·The Journal of Antimicrobial Chemotherapy·Jaeeun KimJeyong Yoon
Apr 14, 2010·Antimicrobial Agents and Chemotherapy·Peter SuciMark Young
Sep 28, 2010·Applied and Environmental Microbiology·Jee Yeon KimJeyong Yoon
May 11, 2010·Canadian Journal of Microbiology·Fabrizio RivardoHoward Ceri
Jun 6, 2009·Future Microbiology·Isabelle PagnierBernard La Scola
Aug 10, 2013·Nanotoxicology·Matthew C StensbergMaria S Sepúlveda
Mar 4, 2015·ACS Applied Materials & Interfaces·Maria Godoy-GallardoMarkus Haapasalo
Apr 3, 2009·International Journal of Antimicrobial Agents·Douglas Roberto MonteiroDebora Barros Barbosa
Aug 14, 2012·Biotechnology and Bioengineering·Yanyan Zhang, Zhiqiang Hu
Oct 22, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Indu BajpaiBikramjit Basu
Aug 27, 2015·ACS Nano·Melek KiristiJoseph Wang
Apr 29, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M D MaciàA Oliver
Jan 15, 2015·Biomacromolecules·Hyun-Su LeeRussell J Composto
Mar 5, 2013·International Journal of Medical Microbiology : IJMM·Frida MarianaThomas Maskow
Apr 24, 2010·FEMS Immunology and Medical Microbiology·Iolanda Francolini, Gianfranco Donelli
Apr 4, 2012·FEMS Immunology and Medical Microbiology·Luanne Hall-StoodleyThomas Bjarnsholt
Jul 30, 2016·Veterinary and Comparative Orthopaedics and Traumatology : V.C.O.T·Michael G FicklinMichael P Kowaleski
Aug 28, 2014·ACS Applied Materials & Interfaces·Sudeep GoswamiAiyagari Ramesh
Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Oana CiofuAntonio Oliver
Jul 29, 2011·Nanomedicine·Matthew Charles StensbergMaría Soledad Sepúlveda

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