Comparison of the bead-based simultaneous analysis of specific platelet antibodies assay (SASPA) and Pak Lx Luminex technology with the monoclonal antibody immobilization of platelet antigens assay (MAIPA) to detect platelet alloantibodies

Clinical Chemistry and Laboratory Medicine : CCLM
Lisa RockenbauerSimon Panzer

Abstract

The identification of platelet alloantibodies is indispensible for the diagnoses of fetal/neonatal alloimmune thrombocytopenia and refractoriness to platelet transfusions. We compared the results obtained with the gold-standard assay for the detection and specification of platelet antibodies, the monoclonal antibody-specific immobilization of platelet antigen assay (MAIPA), with those from two bead-based assays, simultaneous analysis of specific platelet antibodies assay (SASPA), and the recently commercialized bead-based assay Pak Lx. For the detection of alloantibodies by SASPA, the sensitivity was 94.6%, the specificity was 99.5% The respective results for Pak Lx were 98% and 99.8%. Some antibody specificities were revealed by SASPA or Pak Lx but not by MAIPA. However, there were also antibodies detected by MAIPA that were not seen by SASPA or Pak Lx. Antibody dilution experiments suggest that SASPA or Pak Lx are more sensitive than MAIPA. All three assays have limitations, but Pak Lx is suited for screening. However, other assays are necessary if results are not conclusive or for evaluations beyond the commercial assay's design.

References

Sep 1, 1992·Transfusion Medicine·V Kiefel
Nov 30, 2004·British Journal of Haematology·Xuan Duc NguyenHarald Klüter
Dec 12, 2007·Vox Sanguinis·C KaplanUNKNOWN International Society of Blood Transfusion--Working Party on Platelet Immunology
Feb 7, 2013·British Journal of Haematology·Julie A PetersonRichard H Aster
Jun 13, 2013·American Journal of Hematology·Nahla M Heikal, Kristi J Smock

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Citations

Aug 7, 2019·Journal of Translational Medicine·Sudan TaoFaming Zhu
Apr 6, 2021·Hematology, Transfusion and Cell Therapy·Valeria de Freitas DutraCarolina Bonet Bub

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Methods Mentioned

BETA
ELISA
genotyping
antigen capture

Software Mentioned

MAIPA
SASPA
Pak

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