PMID: 11912054Mar 26, 2002Paper

Comparison of the biodistribution of free or liposome-entrapped Crotalus durissus terrificus (South American rattlesnake) venom in mice

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
R T GomesT V Freitas

Abstract

The local absorption rate, clearance and tissue distribution of Crotalus durissus terrificus venom, (Cdt) were examined using a two-antibody sandwich ELISA assay. We compared the biodistribution of both free or encapsulated Cdt in mice. Following subcutaneous injection of 10 microg/mouse of free Cdt (0.8 LD50), venom was detected in serum after 15 min, showed its highest level at 30 min (45+/-5 ng/ml) and was cleared from the circulation after 6 h. After 2 h of inoculation, venom was detected in the kidney (57+/-9 ng/g of tissue), spleen (18+/-4 ng/g of tissue) and brain (14+/-6 ng/g of tissue). For both subcutaneous or intravenous injection of free Cdt, venom was firstly detected in the kidney. No Cdt appeared either in the kidney, spleen, brain, or other tissues after subcutaneous inoculation of encapsulated venom even though a higher dose was used, 25 microg/mouse (2 LD50). Venom remained at the site of injection for a period of 1 week. Following intravenous injection of encapsulated venom (5 microg/mouse, 2 LD50), venom was detected in liver and spleen tissues. The biodistribution of encapsulated venom is discussed in relation to the effects of reduction of toxicity and increase of adjuvanticity.

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Citations

Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Simone Cristina YamasakiPaulo Flávio Silveira
Mar 21, 2007·Toxicon : Official Journal of the International Society on Toxinology·Fernanda P B NunesMaria Cristina C Sousa-e-Silva
Jun 15, 2011·Journal of Biochemical and Molecular Toxicology·Jonas Golart da SilvaAlexandra Acco
Nov 23, 2010·Toxicon : Official Journal of the International Society on Toxinology·Juliana Marton BaronePaulo Flávio Silveira
Oct 10, 2012·Experimental Biology and Medicine·Tatiane S LimaMaria Cristina Cirillo
Mar 1, 2020·Journal of Veterinary Emergency and Critical Care·Maximilian Estevan OliveiraYoko Oshima-Franco

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